BMRB: 6089

1H Chemical Shift Assignments of non-specific Lipid Transfer Protein 1 Isolated from Seeds of Mung Bean

Authors

Yu-Nan, L., Shang-Te, H., Dharmaraj, S., Ku-Feng, L., Alexandre, B.M.J.J., Ping-Chiang, L.

Assembly

Lipid transfer protein 1

Entity

1. Lipid transfer protein 1 (polymer, Thiol state: all disulfide bound), 91 monomers, 9298.652 Da Detail

MTCGQVQGNL AQCIGFLQKG GVVPPSCCTG VKNILNSSRT TADRRAVCSC LKAAAGAVRG INPNNAEALP GKCGVNIPYK ISTSTNCNSI N

Formula weight

9298.652 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS50:SG
2	disulfide	sing	1:CYS13:SG	1:CYS27:SG
3	disulfide	sing	1:CYS28:SG	1:CYS73:SG
4	disulfide	sing	1:CYS48:SG	1:CYS87:SG

Source organism

Vigna radiata

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.8 %, Completeness (bb): 99.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	94.8 % (474 of 500)	94.8 % (474 of 500)
Backbone	99.5 % (186 of 187)	99.5 % (186 of 187)
Sidechain	92.0 % (288 of 313)	92.0 % (288 of 313)
Aromatic	55.6 % (5 of 9)	55.6 % (5 of 9)
Methyl	100.0 % (51 of 51)	100.0 % (51 of 51)

1. non-specific lipid transfer protein 1

MTCGQVQGNL AQCIGFLQKG GVVPPSCCTG VKNILNSSRT TADRRAVCSC LKAAAGAVRG INPNNAEALP GKCGVNIPYK ISTSTNCNSI N

Show sample condition

Sample

Temperature 298 (±1) K, pH 3.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	non-specific lipid transfer protein 1			3 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1SIY, Strand ID: A Detail

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Release date

2004-02-02

Citation

Characterization and structural analyses of nonspecific lipid transfer protein 1 from mung bean
Lin, K., Liu, Y., Hsu, S., Samuel, D., Cheng, C.S., Bonvin, A.M.J.J., Lyu, P.
Biochemistry (2005), 44, 5703-5712, PubMed 15823028 , DOI 10.1021/bi047608v , Abstract

Related entities 1. Lipid transfer protein 1, : 1 : 1 : 214 entities Detail

Experiments performed 2 experiments Detail