BMRB: 6090

1H, 13C and 15N resonance assignement of the reduced form of methionine sulfoxide reductase A from Escherichia coli

Authors

Coudevylle, N., Thureau, A., Azza, S., Boshi-Muller, S., Branlant, G., Cung, M.

Assembly

methionine sulfoxide reductase A

Entity

1. methionine sulfoxide reductase A (polymer, Thiol state: all free), 211 monomers, 23183.57 Da Detail

SLFDKKHLVS PADALPGRNT PMPVATLHAV NGHSMTNVPD GMEIAIFAMG CFWGVERLFW QLPGVYSTAA GYTGGYTPNP TYREVCSGDT GHAEAVRIVY DPSVISYEQL LQVFWENHDP AQGMRQGNDH GTQYRSAIYP LTPEQDAAAR ASLERFQAAM LAADDDRHIT TEIANATPFY YAEDDHQQYL HKNPYGYCGI GGIGVCLPPE A

Formula weight

23183.57 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 68.4 %, Completeness (bb): 93.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	68.4 % (1610 of 2354)	60.5 % (732 of 1209)	74.2 % (689 of 929)	87.5 % (189 of 216)
Backbone	93.3 % (1151 of 1234)	91.3 % (388 of 425)	94.0 % (577 of 614)	95.4 % (186 of 195)
Sidechain	48.2 % (632 of 1312)	43.6 % (342 of 784)	56.8 % (288 of 507)	9.5 % (2 of 21)
Aromatic	7.3 % (18 of 246)	8.9 % (11 of 123)	5.8 % (7 of 120)	0.0 % (0 of 3)
Methyl	81.4 % (171 of 210)	79.0 % (83 of 105)	83.8 % (88 of 105)

1. Methionine sulfoxide reductase A

SLFDKKHLVS PADALPGRNT PMPVATLHAV NGHSMTNVPD GMEIAIFAMG CFWGVERLFW QLPGVYSTAA GYTGGYTPNP TYREVCSGDT GHAEAVRIVY DPSVISYEQL LQVFWENHDP AQGMRQGNDH GTQYRSAIYP LTPEQDAAAR ASLERFQAAM LAADDDRHIT TEIANATPFY YAEDDHQQYL HKNPYGYCGI GGIGVCLPPE A

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Methionine sulfoxide reductase A	[U-99% 13C; U-99% 15N]		0.9 mM

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LACS Plot; CA

Referencing offset: -0.55 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.55 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.36 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 2GT3, Strand ID: A Detail

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Release date

2004-02-19

Citation

Letter to the Editor: 1H, 13C and 15N resonance assignement of the reduced form of methionine sulfoxide reductase A from Escherichia coli
Coudevylle, N., Thureau, A., Azza, S., Boshi-Muller, S., Branlant, G., Cung, M.
J. Biomol. NMR (2004), 30, 363-364

Related entities 1. methionine sulfoxide reductase A, : 1 : 2 : 13 : 93 entities Detail

Interaction partners 1. methionine sulfoxide reductase A, : 4 interactors Detail

Experiments performed 9 experiments Detail