BMRB: 6095

Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb

Authors

Zor, T., De Guzman, R.N., Dyson, H., Wright, P.E.

Assembly

CBP KIX domain and c-Myb

Entity

1. CBP KIX domain (polymer, Thiol state: not present), 87 monomers, 10331.68 Da Detail

GVRKGWHEHV TQDLRSHLVH KLVQAIFPTP DPAALKDRRM ENLVAYAKKV EGDMYESANS RDEYYHLLAE KIYKIQKELE EKRRSRL

2. C-MYB (polymer, Thiol state: not present), 25 monomers, 2942.428 Da Detail

KEKRIKELEL LLMSTENELK GQQAL

Total weight

13274.107 Da

Max. entity weight

10331.68 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

initial structures from DYANA and refined in AMBER

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 80.4 %, Completeness: 65.6 %, Completeness (bb): 66.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	65.6 % (909 of 1385)	68.6 % (504 of 735)	59.0 % (314 of 532)	77.1 % (91 of 118)
Backbone	66.5 % (443 of 666)	78.2 % (176 of 225)	54.8 % (182 of 332)	78.0 % (85 of 109)
Sidechain	66.5 % (550 of 827)	64.3 % (328 of 510)	70.1 % (216 of 308)	66.7 % (6 of 9)
Aromatic	61.0 % (50 of 82)	61.0 % (25 of 41)	60.0 % (24 of 40)	100.0 % (1 of 1)
Methyl	71.3 % (87 of 122)	72.1 % (44 of 61)	70.5 % (43 of 61)

1. CBP KIX domain

GVRKGWHEHV TQDLRSHLVH KLVQAIFPTP DPAALKDRRM ENLVAYAKKV EGDMYESANS RDEYYHLLAE KIYKIQKELE EKRRSRL

2. C-MYB

KEKRIKELEL LLMSTENELK GQQAL

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CBP KIX domain	[U-N15; U-C13]		0.5 ~ 1.0 mM
2	C-MYB	[U-N15; U-C13]		0.5 ~ 1.0 mM

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Chem. Shift Complete2

Sequence coverage: 38.4 %, Completeness: 46.8 %, Completeness (bb): 48.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	46.8 % (1295 of 2770)	48.3 % (710 of 1470)	42.5 % (452 of 1064)	56.4 % (133 of 236)
Backbone	48.0 % (639 of 1332)	55.3 % (249 of 450)	40.2 % (267 of 664)	56.4 % (123 of 218)
Sidechain	47.0 % (778 of 1654)	45.3 % (462 of 1020)	49.7 % (306 of 616)	55.6 % (10 of 18)
Aromatic	30.5 % (50 of 164)	30.5 % (25 of 82)	30.0 % (24 of 80)	50.0 % (1 of 2)
Methyl	50.0 % (122 of 244)	50.0 % (61 of 122)	50.0 % (61 of 122)

1. CBP KIX domain

GVRKGWHEHV TQDLRSHLVH KLVQAIFPTP DPAALKDRRM ENLVAYAKKV EGDMYESANS RDEYYHLLAE KIYKIQKELE EKRRSRL

2. C-MYB

KEKRIKELEL LLMSTENELK GQQAL

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CBP KIX domain	[U-N15; U-C13]		0.5 ~ 1.0 mM
2	C-MYB	[U-N15; U-C13]		0.5 ~ 1.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1SB0, Strand ID: A, B Detail

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Release date

2004-05-14

Citation

Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb
Zor, T., De Guzman, R.N., Dyson, H., Wright, P.E.
J. Mol. Biol. (2004), 337, 521-534, PubMed 15019774 , DOI 10.1016/j.jmb.2004.01.038 , Abstract

Related entities 1. CBP KIX domain, : 6 : 23 entities Detail

Related entities 2. C-MYB, : 1 : 8 : 1 : 2 : 9 entities Detail

Interaction partners 1. CBP KIX domain, : 1 interactors Detail

Interaction partners 2. C-MYB, : 2 interactors Detail