BMRB: 6096

The solution structure of the pH-induced monomer of dynein light chain LC8 from Drosophila

Authors

Makokha, M., Huang, Y.J., Montelione, G.T., Edison, A.S., Barbar, E.

Assembly

Dynein light chain 1, cytoplasmic

Entity

1. Dynein light chain 1, cytoplasmic (polymer, Thiol state: all free), 89 monomers, 10373.72 Da Detail

MSDRKAVIKN ADMSEEMQQD AVDCATQALE KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR NFGSYVTHET RHFIYFYLGQ VAILLFKSG

Formula weight

10373.72 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.2 %, Completeness (bb): 81.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.2 % (918 of 1078)	91.3 % (511 of 560)	75.5 % (318 of 421)	91.8 % (89 of 97)
Backbone	81.8 % (435 of 532)	95.0 % (172 of 181)	66.9 % (176 of 263)	98.9 % (87 of 88)
Sidechain	89.9 % (567 of 631)	89.4 % (339 of 379)	93.0 % (226 of 243)	22.2 % (2 of 9)
Aromatic	88.5 % (108 of 122)	98.4 % (60 of 61)	78.3 % (47 of 60)	100.0 % (1 of 1)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. dynein light chain

MSDRKAVIKN ADMSEEMQQD AVDCATQALE KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR NFGSYVTHET RHFIYFYLGQ VAILLFKSG

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 3.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	dynein light chain	[U-15N; U-13C]		0.8 ~ 1.4 mM
2	NaCl			50 mM

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LACS Plot; CA

Referencing offset: -0.39 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.39 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 10 models in PDB: 1RHW, Strand ID: A Detail

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Release date

2004-05-14

Citation

The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila
Makokha, M., Huang, Y.J., Montelione, G.T., Edison, A.S., Barbar, E.
Protein Sci. (2004), 13, 727-734, PubMed 14767079 , DOI 10.1110/ps.03462204 , Abstract

Related entities 1. Dynein light chain 1, cytoplasmic, : 1 : 16 : 13 : 3 : 195 entities Detail

Interaction partners 1. Dynein light chain 1, cytoplasmic, : 44 interactors Detail

Experiments performed 10 experiments Detail