BMRB: 6103

Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha

Authors

Weljie, A.M., Vogel, H.J.

Assembly

Calcium-dependent protein kinase SK5 (E.C.2.7.1.-)

Entity

1. Calcium-dependent protein kinase SK5 (polymer, Thiol state: not present), 87 monomers, 9629.495 Da Detail

HSSGHIDDDD KHMAERLSEE EIGGLKELFK MIDTDNSGTI TFDELKDGLK RVGSELMESE IKDLMDAADI DKSGTIDYGE FIAATVH

2. CA (non-polymer), 40.078 × 2 Da

Total weight

9709.651 Da

Max. entity weight

9629.495 Da

Source organism

Glycine max

Exptl. method

NMR

Refine. method

simulated annealing; molecular dynamics; matrix relaxation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 70.3 %, Completeness (bb): 87.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	70.3 % (675 of 960)	76.8 % (380 of 495)	56.8 % (214 of 377)	92.0 % (81 of 88)
Backbone	87.5 % (457 of 522)	90.7 % (165 of 182)	83.4 % (211 of 253)	93.1 % (81 of 87)
Sidechain	54.4 % (281 of 517)	68.7 % (215 of 313)	32.5 % (66 of 203)	0.0 % (0 of 1)
Aromatic	22.2 % (12 of 54)	44.4 % (12 of 27)	0.0 % (0 of 27)
Methyl	42.0 % (37 of 88)	75.0 % (33 of 44)	9.1 % (4 of 44)

1. Calcium-dependent protein kinase SK5

HSSGHIDDDD KHMAERLSEE EIGGLKELFK MIDTDNSGTI TFDELKDGLK RVGSELMESE IKDLMDAADI DKSGTIDYGE FIAATVH

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	Calcium-dependent protein kinase SK5	[U-15N]		0.5 mM
2	NaCl	natural abundance		200 mM
3	CaCl2	natural abundance		10 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

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LACS Plot; CA

Referencing offset: -0.41 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
13N	DSS	methyl protons	0.0 ppm	null	null	0.25
15C	DSS	methyl protons	0.0 ppm	null	null	0.1

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LACS Plot; CB

Referencing offset: -0.41 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
13N	DSS	methyl protons	0.0 ppm	null	null	0.25
15C	DSS	methyl protons	0.0 ppm	null	null	0.1

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
13N	DSS	methyl protons	0.0 ppm	null	null	0.25
15C	DSS	methyl protons	0.0 ppm	null	null	0.1

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LACS Plot; CO

Referencing offset: -0.7 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
13N	DSS	methyl protons	0.0 ppm	null	null	0.25
15C	DSS	methyl protons	0.0 ppm	null	null	0.1

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Protein Blocks Logo

Calculated from 15 models in PDB: 1S6J, Strand ID: A Detail

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Release date

2005-02-28

Citation

Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha
Weljie, A.M., Vogel, H.J.
J. Biol. Chem. (2004), 279, 35494-35502, PubMed 15155727 , DOI 10.1074/jbc.M311520200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. Calcium-dependent protein kinase SK5, : 1 : 1 : 453 entities Detail

Experiments performed 1 experiments Detail