BMRBj - BMREntryMaster

Found 1 Document (1.976 seconds)

BMRB: 6104

1S6I

Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha

Authors

Weljie, A.M., Vogel, H.J.

Assembly

Calcium-dependent protein kinase SK5 (E.C.2.7.1.-)

Entity

1. Calcium-dependent protein kinase SK5 (polymer, Thiol state: not present), 188 monomers, 21229.38 Da Detail

AERLSEEEIG GLKELFKMID TDNSGTITFD ELKDGLKRVG SELMESEIKD LMDAADIDKS GTIDYGEFIA ATVHLNKLER EENLVSAFSY FDKDGSGYIT LDEIQQACKD FGLDDIHIDD MIKEIDQDND GQIDYGEFAA MMRKRKGNGG IGRRTMRKTL NLRDALGLVD NGSNQVIEGY FKHHHHHH

2. CA (non-polymer), 40.078 × 4 Da

Total weight

21389.693 Da

Max. entity weight

21229.38 Da

Source organism

Glycine max

Exptl. method

NMR

Refine. method

simulated annealing; molecular dynamics; matrix relaxation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.2 %, Completeness: 70.0 %, Completeness (bb): 78.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.0 % (1507 of 2152)	69.9 % (783 of 1120)	67.5 % (561 of 831)	81.1 % (163 of 201)
Backbone	78.5 % (886 of 1128)	80.3 % (317 of 395)	75.6 % (412 of 545)	83.5 % (157 of 188)
Sidechain	63.9 % (762 of 1193)	64.3 % (466 of 725)	63.7 % (290 of 455)	46.2 % (6 of 13)
Aromatic	21.1 % (32 of 152)	42.1 % (32 of 76)	0.0 % (0 of 76)
Methyl	82.1 % (151 of 184)	80.4 % (74 of 92)	83.7 % (77 of 92)

1. Calcium-dependent protein kinase SK5

Show sample condition

Sample

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
1	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.8 mM
2	unlabelled JD peptide	natural abundance	0.9 mM
3	H2-Imidazole	natural abundance	50 mM
4	NaCl	natural abundance	150 mM
5	CaCl2	natural abundance	10 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	na	null	null	0.0
¹³C	DSS	na	null	null	0.0

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	na	null	null	0.0
¹³C	DSS	na	null	null	0.0

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	na	null	null	0.0
¹³C	DSS	na	null	null	0.0

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	na	null	null	0.0
¹³C	DSS	na	null	null	0.0

Referencing offset: -0.79 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1S6I, Strand ID: A Detail

Release date

2005-02-28

Citation

Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha
Weljie, A.M., Vogel, H.J.
J. Biol. Chem. (2004), 279, 35494-35502, PubMed 15155727 , DOI 10.1074/jbc.M311520200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 6103 released on 2005-02-28
    Title Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha
  Swiss-Prot: P28583 released on 1992-12-01
    Title CDPK_SOYBN Entity Calcium-dependent protein kinase SK5

Related entities 1. Calcium-dependent protein kinase SK5, : 1 : 106 entities Detail

Experiments performed 2 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
1	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.8 mM
2	unlabelled JD peptide	natural abundance	0.9 mM
3	H2-Imidazole	natural abundance	50 mM
4	NaCl	natural abundance	150 mM
5	CaCl2	natural abundance	10 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
8	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.5 mM
9	unlabelled JD peptide	natural abundance	0.6 mM
10	H2-Imidazole	natural abundance	50 mM
11	NaCl	natural abundance	200 mM
12	CaCl2	natural abundance	10 mM
13	H2O	natural abundance	95 %
14	D2O	natural abundance	5 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
15	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.4 mM
16	unlabelled JD peptide	natural abundance	0.5 mM
17	H2-Imidazole	natural abundance	50 mM
18	NaCl	natural abundance	200 mM
19	CaCl2	natural abundance	10 mM
20	H2O	natural abundance	95 %
21	D2O	natural abundance	5 %

2 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
1	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.8 mM
2	unlabelled JD peptide	natural abundance	0.9 mM
3	H2-Imidazole	natural abundance	50 mM
4	NaCl	natural abundance	150 mM
5	CaCl2	natural abundance	10 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
8	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.5 mM
9	unlabelled JD peptide	natural abundance	0.6 mM
10	H2-Imidazole	natural abundance	50 mM
11	NaCl	natural abundance	200 mM
12	CaCl2	natural abundance	10 mM
13	H2O	natural abundance	95 %
14	D2O	natural abundance	5 %

Sample #3

Pressure 1 atm, Temperature 313 K, pH 7.3

#	Name	Isotope labeling	Concentration
15	Calcium-dependent protein kinase SK5	[U-15N; U-13C]	0.4 mM
16	unlabelled JD peptide	natural abundance	0.5 mM
17	H2-Imidazole	natural abundance	50 mM
18	NaCl	natural abundance	200 mM
19	CaCl2	natural abundance	10 mM
20	H2O	natural abundance	95 %
21	D2O	natural abundance	5 %

nullKeywords EF-hand, helix-loop-helix, calcium-binding, calmodulin superfamily

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Found 1 Document (1.976 seconds)

BMRB: 6104

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. Calcium-dependent protein kinase SK5, : 1 : 106 entities Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3