BMRB: 6108

Solution structure of GlgS protein from E. coli

Authors

Kozlov, G., Gehring, K.

Assembly

Glycogen synthesis protein glgS

Entity

1. Glycogen synthesis protein glgS (polymer, Thiol state: all free), 86 monomers, 10055.11 Da Detail

MGSSHHHHHH SSGLVPRGSH MDHSLNSLNN FDFLARSFAR MHAEGRPVDI LAVTGNMDEE HRTWFCARYA WYCQQMMQAR ELELEH

Formula weight

10055.11 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 79.1 %, Completeness: 64.8 %, Completeness (bb): 75.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	64.8 % (641 of 989)	73.6 % (377 of 512)	51.8 % (199 of 384)	69.9 % (65 of 93)
Backbone	75.8 % (388 of 512)	76.0 % (133 of 175)	75.1 % (190 of 253)	77.4 % (65 of 84)
Sidechain	56.5 % (315 of 558)	72.4 % (244 of 337)	33.5 % (71 of 212)	0.0 % (0 of 9)
Aromatic	31.5 % (39 of 124)	54.8 % (34 of 62)	8.3 % (5 of 60)	0.0 % (0 of 2)
Methyl	58.1 % (36 of 62)	87.1 % (27 of 31)	29.0 % (9 of 31)

1. glgS

MGSSHHHHHH SSGLVPRGSH MDHSLNSLNN FDFLARSFAR MHAEGRPVDI LAVTGNMDEE HRTWFCARYA WYCQQMMQAR ELELEH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	glgS	[U-13C; U-15N]		1 mM
2	potassium phosphate			5 mM
3	DTT			1 mM
4	sodium azide			0.1 mM
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 15 models in PDB: 1RRZ, Strand ID: A Detail

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Release date

2004-06-29

Citation

Structure of GlgS from Escherichia coli suggests a role in protein-protein interactions
Kozlov, G., Elias, D., Cygler, M., Gehring, K.
BMC Biol. (2004), 2, 10-10, PubMed 15161493 , DOI 10.1186/1741-7007-2-10 , Abstract

Related entities 1. Glycogen synthesis protein glgS, : 1 : 13 entities Detail

Experiments performed 2 experiments Detail