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BMRB: 6111


6111
1Z2F
1H, 13C and 15N resonance assignments of the antifreeze protein cfAFP-501 from spruce budworm at different temperatures
Authors
Li, C., Jin, C.
Assembly
Choristoneura fumiferana antifreeze protein isoform 501
Entity
1. Choristoneura fumiferana antifreeze protein isoform 501 (polymer, Thiol state: all disulfide bound), 121 monomers, 12538.82 × 2 Da Detail

DGTCVNTNSQ ITANSQCVKS TATNCYIDNS QLVDTSICTR SQYSDANVKK SVTTDCNIDK SQVYLTTCTG SQYNGIYIRS STTTGTSISG PGCSISTCTI TRGVATPAAA CKISGCSLSA M


Total weight
25077.64 Da
Max. entity weight
12538.82 Da
Entity Connection
disulfide 10 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS4:SG1:CYS17:SG
2disulfidesing1:CYS25:SG1:CYS38:SG
3disulfidesing1:CYS56:SG1:CYS68:SG
4disulfidesing1:CYS93:SG1:CYS116:SG
5disulfidesing1:CYS98:SG1:CYS111:SG
6disulfidesing1:CYS4:SG1:CYS17:SG
7disulfidesing1:CYS25:SG1:CYS38:SG
8disulfidesing1:CYS56:SG1:CYS68:SG
9disulfidesing1:CYS93:SG1:CYS116:SG
10disulfidesing1:CYS98:SG1:CYS111:SG

Source organism
Choristoneura fumiferana
Exptl. method
NMR
Refine. method
simulated annealing, molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 89.6 %, Completeness (bb): 96.5 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All89.6 % (1113 of 1242)88.2 % (554 of 628)89.8 % (432 of 481)95.5 % (127 of 133)
Backbone96.5 % (697 of 722)96.4 % (239 of 248)95.8 % (340 of 355)99.2 % (118 of 119)
Sidechain83.1 % (526 of 633)82.9 % (315 of 380)84.5 % (202 of 239)64.3 % (9 of 14)
Aromatic20.0 % (8 of 40)20.0 % (4 of 20)20.0 % (4 of 20)
Methyl75.7 % (103 of 136)73.5 % (50 of 68)77.9 % (53 of 68)

1. Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501

DGTCVNTNSQ ITANSQCVKS TATNCYIDNS QLVDTSICTR SQYSDANVKK SVTTDCNIDK SQVYLTTCTG SQYNGIYIRS STTTGTSISG PGCSISTCTI TRGVATPAAA CKISGCSLSA M

Show sample condition
Sample

Temperature 290 (±0.1) K, pH 5.7 (±0.2)


#NameIsotope labelingTypeConcentration
1Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501[U-15N; U-13C]0.25 ~ 0.5 mM

Chem. Shift Complete2
Sequence coverage: 99.2 %, Completeness: 72.7 %, Completeness (bb): 90.7 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All72.7 % (1807 of 2484)68.3 % (858 of 1256)73.2 % (704 of 962)92.1 % (245 of 266)
Backbone90.7 % (1310 of 1444)96.4 % (478 of 496)84.2 % (598 of 710)98.3 % (234 of 238)
Sidechain49.8 % (630 of 1266)50.0 % (380 of 760)50.0 % (239 of 478)39.3 % (11 of 28)
Aromatic15.0 % (12 of 80)20.0 % (8 of 40)10.0 % (4 of 40)
Methyl46.3 % (126 of 272)44.1 % (60 of 136)48.5 % (66 of 136)

1. Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501

DGTCVNTNSQ ITANSQCVKS TATNCYIDNS QLVDTSICTR SQYSDANVKK SVTTDCNIDK SQVYLTTCTG SQYNGIYIRS STTTGTSISG PGCSISTCTI TRGVATPAAA CKISGCSLSA M

Show sample condition
Sample

Temperature 270 (±0.1) K, pH 5.7 (±0.2)


#NameIsotope labelingTypeConcentration
1Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501[U-15N; U-13C]0.25 ~ 0.5 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1Z2F, Strand ID: A Detail

Release date
2004-02-22
Citation 1
(1)H, (13)C and (15)N resonance assignments of the antifreeze protein cfAFP-501 from spruce budworm at different temperatures
Li, C., Jin, C.
J. Biomol. NMR (2004), 30, 101-102, PubMed 15452439 , DOI 10.1023/B:JNMR.0000042949.59771.69
Show more 1 citaion
Citation 2
Solution structure of an antifreeze protein CfAFP-501 from Choristoneura fumiferana
Li, C., Guo, X., Jia, Z., Xia, B., Jin, C.
J. Biomol. NMR (2005), 32, 251-256, PubMed 16132825 , DOI 10.1007/s10858-005-8206-3 ,
Related entities 1. Choristoneura fumiferana antifreeze protein isoform 501, : 1 : 1 : 5 entities Detail
More details for 7 related entities
Experiments performed 1 experiments Detail
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