BMRB: 6120

Backbone 1H, 13C, and 15N Chemical Shift Assignments for NESG target CcR19

Authors

Shen, Y., Hanudatta, A.S., Xiao, R., Acton, T.B., Szyperski, T.

Assembly

Hypothetical protein CC1736

Entity

1. Hypothetical protein CC1736 (polymer, Thiol state: not present), 148 monomers, 16663.93 Da Detail

MHRHVVTKVL PYTPDQLFEL VGDVDAYPKF VPWITGMRTW NGRVDGAVST VDAEAQVGFS FLREKFATRV RRDKDARSID VSLLYGPFKR LNNGWRFMPE GDATRVEFVI EFAFKSALLD AMLAANVDRA AGKLIACFEA RAQQLHGA

Formula weight

16663.93 Da

Source organism

Caulobacter vibrioides CB15

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 88.8 %, Completeness (bb): 94.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.8 % (1537 of 1731)	90.3 % (806 of 893)	86.0 % (589 of 685)	92.8 % (142 of 153)
Backbone	94.7 % (830 of 876)	96.7 % (290 of 300)	93.8 % (407 of 434)	93.7 % (133 of 142)
Sidechain	84.7 % (841 of 993)	87.0 % (516 of 593)	81.2 % (316 of 389)	81.8 % (9 of 11)
Aromatic	64.3 % (117 of 182)	79.1 % (72 of 91)	47.7 % (42 of 88)	100.0 % (3 of 3)
Methyl	96.6 % (168 of 174)	97.7 % (85 of 87)	95.4 % (83 of 87)

1. Hypothetical protein CC1736

MHRHVVTKVL PYTPDQLFEL VGDVDAYPKF VPWITGMRTW NGRVDGAVST VDAEAQVGFS FLREKFATRV RRDKDARSID VSLLYGPFKR LNNGWRFMPE GDATRVEFVI EFAFKSALLD AMLAANVDRA AGKLIACFEA RAQQLHGA

Show sample condition

Sample

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical protein CC1736	[U-13C; U-15N]		1.1 mM
2	D2O			5 %
3	NaN3			0.02 %
4	DTT			10 mM
5	CaCl2			5 mM
6	NaCl			100 mM
7	MES			20 mM

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.13 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1T17, Strand ID: A Detail

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Release date

2004-07-22

Citation

Complete genome sequence of Caulobacter crescentus
Shen, Y., Hanudatta, A.S., Xiao, R., Acton, T.B., Shastry, R., Ma, L., Montelione, G.T., Szyperski, T.
Proc. Natl. Acad. Sci. U. S. A. (2001), 98, 4136-4141, PubMed 11259647 , DOI 10.1073/pnas.061029298 , Abstract

Related entities 1. Hypothetical protein CC1736, : 1 : 42 entities Detail

Experiments performed 8 experiments Detail