BMRB: 6126

NMR Structure of the CRIB-PDZ module of Par-6

Authors

Peterson, F.C., R Penkert, R., Volkman, B.F., Prehoda, K.E.

Assembly

CG5884-PA

Entity

1. CG5884-PA (polymer, Thiol state: not present), 128 monomers, 13741.60 Da Detail

GSKTKAPSIS IPHDFRQVSA IIDVDIVPET HRRVRLLKHG SDKPLGFYIR DGTSVRVTAS GLEKQPGIFI SRLVPGGLAE STGLLAVNDE VIEVNGIEVA GKTLDQVTDM MVANSSNLII TVKPANQR

Formula weight

13741.6 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 93.1 %, Completeness (bb): 97.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (1341 of 1440)	91.3 % (680 of 745)	94.7 % (535 of 565)	96.9 % (126 of 130)
Backbone	97.9 % (738 of 754)	97.7 % (254 of 260)	98.4 % (367 of 373)	96.7 % (117 of 121)
Sidechain	89.5 % (719 of 803)	87.8 % (426 of 485)	91.9 % (284 of 309)	100.0 % (9 of 9)
Aromatic	80.0 % (40 of 50)	96.0 % (24 of 25)	64.0 % (16 of 25)
Methyl	92.6 % (163 of 176)	90.9 % (80 of 88)	94.3 % (83 of 88)

1. Par-6

GSKTKAPSIS IPHDFRQVSA IIDVDIVPET HRRVRLLKHG SDKPLGFYIR DGTSVRVTAS GLEKQPGIFI SRLVPGGLAE STGLLAVNDE VIEVNGIEVA GKTLDQVTDM MVANSSNLII TVKPANQR

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	Par-6	[U-15N; U-13C]		1 mM
2	phosphate buffer			20 mM
3	sodium chloride			50 mM
4	sodium azide			0.05 %
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.39 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.39 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1RY4, Strand ID: A Detail

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Release date

2004-05-14

Citation

Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition
Peterson, F.C., Penkert, R.R., Volkman, B.F., Prehoda, K.E.
Mol. Cell (2004), 13, 665-676, PubMed 15023337 , DOI: , Abstract

Related entities 1. CG5884-PA, : 1 : 2 : 75 entities Detail

Experiments performed 3 experiments Detail