BMRB: 6127

Solution structure of the E. coli bacteriophage P1 encoded HOT protein: a homologue of the theta subunit of E. coli DNA polymerase III

Authors

DeRose, E.F., Kirby, T.W., Mueller, G.A., Chikova, A.K., Schaaper, R.M., London, R.E.

Assembly

p1 encoded protein

Entity

1. p1 encoded protein (polymer, Thiol state: not present), 83 monomers, 9694.844 Da Detail

MYDWNIAAKS QEERDKVNVD LAASGVAYKE RLNIPVIAEQ VAREQPENLR TYFMERLRHY RQLSLQLPKG SDPAYQKDDA VKK

Formula weight

9694.844 Da

Source organism

Escherichia virus P1

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 83.1 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.1 % (833 of 1003)	76.1 % (404 of 531)	89.8 % (343 of 382)	95.6 % (86 of 90)
Backbone	95.5 % (468 of 490)	93.9 % (154 of 164)	96.4 % (238 of 247)	96.2 % (76 of 79)
Sidechain	74.9 % (445 of 594)	68.1 % (250 of 367)	85.6 % (185 of 216)	90.9 % (10 of 11)
Aromatic	57.6 % (38 of 66)	57.6 % (19 of 33)	56.3 % (18 of 32)	100.0 % (1 of 1)
Methyl	86.9 % (73 of 84)	85.7 % (36 of 42)	88.1 % (37 of 42)

1. p1 encoded protein

MYDWNIAAKS QEERDKVNVD LAASGVAYKE RLNIPVIAEQ VAREQPENLR TYFMERLRHY RQLSLQLPKG SDPAYQKDDA VKK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	p1 encoded protein	[U-13C; U-15N]		1.3 mM
2	Tris			5 mM
3	NaCl			100 mM
4	NaN3			5 mM
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 7 models in PDB: 1SE7, Strand ID: A Detail

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Release date

2005-02-14

Citation

Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase III
DeRose, E.F., Kirby, T.W., Mueller, G.A., Chikova, A.K., Schaaper, R.M., London, R.E.
Structure (2004), 12, 2221-2231, PubMed 15576035 , DOI 10.1016/j.str.2004.09.019 , Abstract

Related entities 1. p1 encoded protein, : 1 : 1 : 1 entities Detail

Experiments performed 2 experiments Detail