BMRBj - BMREntryMaster

Found 1 Document (1.429 seconds)

BMRB: 6129

1S6U

Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A

Authors

Banci, L., Bertini, I., Del Conte, R., D'Onofrio, M., Rosato, A.

Assembly

Copper-transporting ATPase 1 (E.C.3.6.3.4)

Entity

1. ATPase (polymer, Thiol state: not reported), 76 monomers, 8446.875 Da Detail

GEVVLKMKVE GMTCHSCTST IEGKIGKLQG VQRIKVSLDN QEATIVYQPH LISVEEMKKQ IEAMGFPAFV KKIEGR

2. CU (non-polymer), 63.546 Da

Total weight

8510.421 Da

Max. entity weight

8446.875 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.0 %, Completeness (bb): 83.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (766 of 901)	92.4 % (437 of 473)	72.1 % (251 of 348)	97.5 % (78 of 80)
Backbone	83.0 % (375 of 452)	98.7 % (155 of 157)	67.0 % (148 of 221)	97.3 % (72 of 74)
Sidechain	88.8 % (460 of 518)	89.2 % (282 of 316)	87.8 % (172 of 196)	100.0 % (6 of 6)
Aromatic	30.6 % (11 of 36)	61.1 % (11 of 18)	0.0 % (0 of 18)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. ATPase

GEVVLKMKVE GMTCHSCTST IEGKIGKLQG VQRIKVSLDN QEATIVYQPH LISVEEMKKQ IEAMGFPAFV KKIEGR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	COPPER (I) ION	1 mM
3	phosphate	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	ATPase	[U-15N]	1 mM
7	COPPER (I) ION		1 mM
8	phosphate		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	ATPase	[U-13C; U-15N]	1 mM
12	COPPER (I) ION		1 mM
13	phosphate		100 mM
14	H2O		90 %
15	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 2.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 2.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1S6U, Strand ID: A Detail

Release date

2004-05-14

Citation

Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A
Banci, L., Bertini, I., Del Conte, R., D'Onofrio, M., Rosato, A.
Biochemistry (2004), 43, 3396-3403, PubMed 15035611 , DOI 10.1021/bi036042s , Abstract

Related entities 1. ATPase, : 1 : 1 : 3 : 282 entities Detail

Interaction partners 1. ATPase, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 4 experiments Detail

1 [1H-1H]-NOESY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	COPPER (I) ION	1 mM
3	phosphate	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	ATPase	[U-15N]	1 mM
7	COPPER (I) ION		1 mM
8	phosphate		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	ATPase	[U-13C; U-15N]	1 mM
12	COPPER (I) ION		1 mM
13	phosphate		100 mM
14	H2O		90 %
15	D2O		10 %

2 13C-NOESY-HSQC

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	COPPER (I) ION	1 mM
3	phosphate	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	ATPase	[U-15N]	1 mM
7	COPPER (I) ION		1 mM
8	phosphate		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	ATPase	[U-13C; U-15N]	1 mM
12	COPPER (I) ION		1 mM
13	phosphate		100 mM
14	H2O		90 %
15	D2O		10 %

3 15N-NOESY-HSQC

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	COPPER (I) ION	1 mM
3	phosphate	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	ATPase	[U-15N]	1 mM
7	COPPER (I) ION		1 mM
8	phosphate		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	ATPase	[U-13C; U-15N]	1 mM
12	COPPER (I) ION		1 mM
13	phosphate		100 mM
14	H2O		90 %
15	D2O		10 %

4 HNHA

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	COPPER (I) ION	1 mM
3	phosphate	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	ATPase	[U-15N]	1 mM
7	COPPER (I) ION		1 mM
8	phosphate		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	ATPase	[U-13C; U-15N]	1 mM
12	COPPER (I) ION		1 mM
13	phosphate		100 mM
14	H2O		90 %
15	D2O		10 %

nullKeywords copper homeostasis, Menkes, metal transport

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Found 1 Document (1.429 seconds)

BMRB: 6129

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. ATPase, : 1 : 1 : 3 : 282 entities Detail

Interaction partners 1. ATPase, : 5 interactors Detail

Experiments performed 4 experiments Detail

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