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Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A
Authors
Banci, L., Bertini, I., Del Conte, R., D'Onofrio, M., Rosato, A.
Assembly
Copper-transporting ATPase 1 (E.C.3.6.3.4)
Entity
1. ATPase (polymer, Thiol state: not reported), 76 monomers, 8446.875 Da Detail

GEVVLKMKVE GMTCHSCTST IEGKIGKLQG VQRIKVSLDN QEATIVYQPH LISVEEMKKQ IEAMGFPAFV KKIEGR


2. CU (non-polymer), 63.546 Da
Total weight
8510.421 Da
Max. entity weight
8446.875 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
restrained energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 85.0 %, Completeness (bb): 83.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All85.0 % (766 of 901)92.4 % (437 of 473)72.1 % (251 of 348)97.5 % (78 of 80)
Backbone83.0 % (375 of 452)98.7 % (155 of 157)67.0 % (148 of 221)97.3 % (72 of 74)
Sidechain88.8 % (460 of 518)89.2 % (282 of 316)87.8 % (172 of 196)100.0 % (6 of 6)
Aromatic30.6 % (11 of 36)61.1 % (11 of 18) 0.0 % (0 of 18)
Methyl100.0 % (90 of 90)100.0 % (45 of 45)100.0 % (45 of 45)

1. ATPase

GEVVLKMKVE GMTCHSCTST IEGKIGKLQG VQRIKVSLDN QEATIVYQPH LISVEEMKKQ IEAMGFPAFV KKIEGR

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1ATPase1 mM
2COPPER (I) ION1 mM
3phosphate100 mM
4H2O90 %
5D2O10 %
Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
6ATPase[U-15N]1 mM
7COPPER (I) ION1 mM
8phosphate100 mM
9H2O90 %
10D2O10 %
Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
11ATPase[U-13C; U-15N]1 mM
12COPPER (I) ION1 mM
13phosphate100 mM
14H2O90 %
15D2O10 %

LACS Plot; CA
Referencing offset: 2.17 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 2.17 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.0 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 30 models in PDB: 1S6U, Strand ID: A Detail


Release date
2004-05-14
Citation
Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A
Banci, L., Bertini, I., Del Conte, R., D'Onofrio, M., Rosato, A.
Biochemistry (2004), 43, 3396-3403, PubMed 15035611 , DOI 10.1021/bi036042s ,
Related entities 1. ATPase, : 1 : 1 : 3 : 282 entities Detail
Interaction partners 1. ATPase, : 5 interactors Detail
Experiments performed 4 experiments Detail
nullKeywords copper homeostasis, Menkes, metal transport