Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:HIS37:NE2 | 2:HEM1:FE |
2 | na | sing | 1:HIS61:NE2 | 2:HEM1:FE |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 79.8 % (398 of 499) | 79.8 % (398 of 499) |
Backbone | 95.8 % (161 of 168) | 95.8 % (161 of 168) |
Sidechain | 71.6 % (237 of 331) | 71.6 % (237 of 331) |
Aromatic | 59.2 % (29 of 49) | 59.2 % (29 of 49) |
Methyl | 82.5 % (33 of 40) | 82.5 % (33 of 40) |
1. Cytochrome b5 Mutant V45H
AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEHLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DRPressure 1 atm, Temperature 293 (±0.1) K, pH 7.00 (±0.01)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cytochrome b5 Mutant V45H | 3.0 mM | ||
2 | PROTOPORPHYRIN IX CONTAINING FE | 3.0 mM | ||
3 | H2O | 90 % | ||
4 | D2O | 10 % | ||
5 | Phosphate buffer | 25 mM |
Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.00 (±0.01)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Cytochrome b5 Mutant V45H | 3.0 mM | ||
7 | PROTOPORPHYRIN IX CONTAINING FE | 3.0 mM | ||
8 | D2O | 100 % | ||
9 | Phosphate buffer | 25 mM |