BMRB: 6131

Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H

Authors

Wu, H., Zhang, Q.

Assembly

Cytochrome b5

Entity

1. Cytochrome b5 Mutant V45H (polymer, Thiol state: not present), 82 monomers, 9499.273 Da Detail

AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEHLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

2. HEM (non-polymer, Thiol state: not present), 616.487 Da

Total weight

10115.761 Da

Max. entity weight

9499.273 Da

Entity Connection

na 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:HIS37:NE2	2:HEM1:FE
2	na	sing	1:HIS61:NE2	2:HEM1:FE

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geomitry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 79.8 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	79.8 % (398 of 499)	79.8 % (398 of 499)
Backbone	95.8 % (161 of 168)	95.8 % (161 of 168)
Sidechain	71.6 % (237 of 331)	71.6 % (237 of 331)
Aromatic	59.2 % (29 of 49)	59.2 % (29 of 49)
Methyl	82.5 % (33 of 40)	82.5 % (33 of 40)

1. Cytochrome b5 Mutant V45H

AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEHLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.00 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	Cytochrome b5 Mutant V45H			3.0 mM
2	PROTOPORPHYRIN IX CONTAINING FE			3.0 mM
3	H2O			90 %
4	D2O			10 %
5	Phosphate buffer			25 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.00 (±0.01)

#	Name	Isotope labeling	Type	Concentration
6	Cytochrome b5 Mutant V45H			3.0 mM
7	PROTOPORPHYRIN IX CONTAINING FE			3.0 mM
8	D2O			100 %
9	Phosphate buffer			25 mM

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Calculated from 30 models in PDB: 1SH4, Strand ID: A Detail

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Release date

2005-03-02

Citation

The comparative study on the solution structures of the oxidized boving microsomal cytochrome b5 and mutant V45H
Wu, H., Zhang, Q.
Protein Sci. (2004), 13, 2161-2169, PubMed , DOI:

Related entities 1. Cytochrome b5 Mutant V45H, : 1 : 1 : 15 : 246 entities Detail

Interaction partners 1. Cytochrome b5 Mutant V45H, : 4 interactors Detail

Experiments performed 3 experiments Detail