BMRB: 6135

Solution structure of conotoxin MrVIB

Authors

Daly, N.L., Ekberg, J.A., Thomas, L., Adams, D.J., Lewis, R.J., Craik, D.J.

Assembly

Mu-O-conotoxin MrVIB

Entity

1. Mu-O-conotoxin MrVIB (polymer, Thiol state: all disulfide bound), 31 monomers, 3412.200 Da Detail

ACSKKWEYCI VPILGFVYCC PGLICGPFVC V

Formula weight

3412.2 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS20:SG
2	disulfide	sing	1:CYS9:SG	1:CYS25:SG
3	disulfide	sing	1:CYS19:SG	1:CYS30:SG

Source organism

Conus marmoreus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 78.0 %, Completeness (bb): 93.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	78.0 % (145 of 186)	78.0 % (145 of 186)
Backbone	93.5 % (58 of 62)	93.5 % (58 of 62)
Sidechain	70.2 % (87 of 124)	70.2 % (87 of 124)
Aromatic	37.5 % (9 of 24)	37.5 % (9 of 24)
Methyl	68.4 % (13 of 19)	68.4 % (13 of 19)

1. MrVIB

ACSKKWEYCI VPILGFVYCC PGLICGPFVC V

Show sample condition

Sample

Pressure 1 atm, Temperature 290 (±1) K, pH 3.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MrVIB			1 mM
2	CD3CN			50 %
3	H2O			40 %
4	D2O			10 %

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Calculated from 20 models in PDB: 1RMK, Strand ID: A Detail

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Release date

2004-03-07

Citation

Structures of muO-conotoxins from Conus marmoreus. I nhibitors of tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels in mammalian sensory neurons
Daly, N.L., Ekberg, J.A., Thomas, L., Adams, D.J., Lewis, R.J., Craik, D.J.
J. Biol. Chem. (2004), 279, 25774-25782, PubMed 15044438 , DOI 10.1074/jbc.M313002200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Mu-O-conotoxin MrVIB, : 1 : 1 : 5 entities Detail

Experiments performed 3 experiments Detail