BMRB: 6137

NMR solution structure of proBnIb of 2S albumin stortage proton from Brassica napus

Authors

Pantoja, D., Bruix, M., Rico, M., Santoro, J.

Assembly

proBnIb monomer

Entity

1. proBnIb monomer (polymer, Thiol state: all disulfide bound), 109 monomers, 12531.12 Da Detail

QPQKCQREFQ QEQHLRACQQ WIRQQLAGSP FSENQWGPQQ GPSLREQCCN ELYQEDQVCV CPTLKQAAKS VRVQGQHGPF QSTRIYQIAK NLPNVCNMKQ IGTCPFIAI

Formula weight

12531.12 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS59:SG
2	disulfide	sing	1:CYS18:SG	1:CYS48:SG
3	disulfide	sing	1:CYS49:SG	1:CYS96:SG
4	disulfide	sing	1:CYS61:SG	1:CYS104:SG

Source organism

Brassica napus

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 85.1 %, Completeness (bb): 97.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.1 % (601 of 706)	85.1 % (601 of 706)
Backbone	97.2 % (210 of 216)	97.2 % (210 of 216)
Sidechain	79.8 % (391 of 490)	79.8 % (391 of 490)
Aromatic	72.7 % (32 of 44)	72.7 % (32 of 44)
Methyl	90.7 % (39 of 43)	90.7 % (39 of 43)

1. Brassica napus

QPQKCQREFQ QEQHLRACQQ WIRQQLAGSP FSENQWGPQQ GPSLREQCCN ELYQEDQVCV CPTLKQAAKS VRVQGQHGPF QSTRIYQIAK NLPNVCNMKQ IGTCPFIAI

Show sample condition

Sample

Temperature 308 (±1) K, pH 3.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Brassica napus			1.2 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 19 models in PDB: 1SM7, Strand ID: A Detail

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Release date

2004-03-07

Citation 1

Solution structure and stability against digestion of rproBnIb, a recombinant 2S albumin from rapeseed: relationship to its allergenic properties
Pantoja-Uceda, D., Palomares, O., Bruix, M., Villalba, M., Rodriguez, R., Rico, M., Santoro, J.
Biochemistry (2004), 43, 16036-16045, PubMed 15609998 , DOI 10.1021/bi048069x , Abstract

Show more 1 citaion

Citation 2

Recombinant pronapin precursor produced in Pichia pastoris displays structural and immunologic equivalent properties to its mature product isolated from rapeseed
Palomares, O., Monsalve, R.I., Rodriguez, R., Villalba, M.
Eur. J. Biochem. (2002), 269, 2538-2545, PubMed 12027892 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. proBnIb monomer, : 1 : 36 entities Detail

Experiments performed 3 experiments Detail