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BMRB: 6150

2POA

1H, 15N and 13C resonance assignments of the Apo Sm14-M20(C62V) protein from Schistosoma mansoni

Authors

Pertinhez, T.A., Sforca, M.L., Alves, A.C., Ramos, C.R., Ho, P.L., Tendler, M., Zanchin, N.I., Spisni, A.

Assembly

fatty acid binding protein

Entity

1. fatty acid binding protein (polymer, Thiol state: not present), 133 monomers, 14843.60 Da Detail

MSSFLGKWKL SESHNFDAVM SKLGVSWATR QIGNTVTPTV TFTMDGDKMT MLTESTFKNL SVTFKFGEEF DEKTSDGRNV KSVVEKNSES KLTQTQVDPK NTTVIVREVD GDTMKTTVTV GDVTAIRNYK RLS

Formula weight

14843.6 Da

Source organism

Schistosoma mansoni

Exptl. method

NMR

Refine. method

torsion angle dynamics using dyana software and further minimization with discover module of the insight package

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.8 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.8 % (1297 of 1529)	80.8 % (637 of 788)	88.1 % (527 of 598)	93.0 % (133 of 143)
Backbone	98.1 % (779 of 794)	98.2 % (267 of 272)	98.2 % (384 of 391)	97.7 % (128 of 131)
Sidechain	74.5 % (641 of 860)	71.9 % (371 of 516)	79.8 % (265 of 332)	41.7 % (5 of 12)
Aromatic	57.5 % (61 of 106)	60.4 % (32 of 53)	52.9 % (27 of 51)	100.0 % (2 of 2)
Methyl	76.7 % (112 of 146)	87.7 % (64 of 73)	65.8 % (48 of 73)

1. FABP from Schistosoma mansoni

MSSFLGKWKL SESHNFDAVM SKLGVSWATR QIGNTVTPTV TFTMDGDKMT MLTESTFKNL SVTFKFGEEF DEKTSDGRNV KSVVEKNSES KLTQTQVDPK NTTVIVREVD GDTMKTTVTV GDVTAIRNYK RLS

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.3 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	FABP from Schistosoma mansoni	[U-13C; U-15N]		0.7 mM
2	Na2H2PO4			50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.64 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.64 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.76 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 17 models in PDB: 2POA, Strand ID: A Detail

Release date

2004-07-28

Citation

1H, 15N and 13C resonance assignments of the apo Sm14-M20(C62V) protein, a mutant of Schistosoma mansoni Sm14
Pertinhez, T.A., Sforca, M.L., Alves, A.C., Ramos, C.R., Ho, P.L., Tendler, M., Zanchin, N.I., Spisni, A.
J. Biomol. NMR (2004), 29, 553-554, PubMed 15243195 , DOI 10.1023/B:JNMR.0000034355.38944.cf , Abstract

Related entities 1. fatty acid binding protein, : 1 : 3 : 264 entities Detail

Experiments performed 12 experiments Detail

1 NHSQC