BMRB: 6151

Structure of Transportan in phospholipid bicellar solution

Authors

Barany-Wallje, E., Andersson, A., Maler, L., Graslund, A.

Assembly

Transportan in bicellar solution with [DMPC]/[DHPC]=0.33

Entity

1. Transportan in bicellar solution with [DMPC]/[DHPC]=0.33 (polymer, Thiol state: not present), 27 monomers, 2710.263 Da Detail

GWTLNSAGYL LGKINLKALA ALAKKIX

Formula weight

2710.263 Da

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 94.9 %, Completeness (bb): 94.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	94.9 % (150 of 158)	94.9 % (150 of 158)
Backbone	94.5 % (52 of 55)	94.5 % (52 of 55)
Sidechain	95.1 % (98 of 103)	95.1 % (98 of 103)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	95.5 % (21 of 22)	95.5 % (21 of 22)

1. Transportan

GWTLNSAGYL LGKINLKALA ALAKKIX

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 5.6

#	Name	Isotope labeling	Type	Concentration
1	Transportan			1 mM
2	[DMPC]/[PHPC]=0.33			300 mM
3	phosphate buffer			50 mM
4	H2O			90 %
5	D2O			10 %

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Calculated from 25 models in PDB: 1SMZ, Strand ID: A Detail

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Release date

2008-07-15

Citation

NMR solution structure and position of transportan in neutral phospholipid bicelles
Barany-Wallje, E., Andersson, A., Graslund, A., Maler, L.
FEBS Lett. (2004), 567, 265-269, PubMed 15178334 , DOI 10.1016/j.febslet.2004.04.079 , Abstract

Related entities 1. Transportan in bicellar solution with [DMPC]/[DHPC]=0.33, : 1 : 6 entities Detail

Experiments performed 2 experiments Detail