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BMRB: 6159

2B1O

1H, 13C and 15N resonance assignments of Ca2+-free DdCAD-1, a cell-cell adhesion molecule

Authors

Lin, Z., Yang, D., Huang, E., Siu, C.

Assembly

DdCAD-1 monomer

Entity

1. DdCAD-1 monomer (polymer, Thiol state: all free), 214 monomers, 23938.43 Da Detail

GSSVDANKVK FFFGKNCTGE SFEYNKGETV RFNNGDKWND KFMSCLVGSN VRCNIWEHNE IDTPTPGKFQ ELAQGSTNND LTSINGLSKF QVLPGAFQWA VDVKIVNKVN STAGSYEMTI TPYQVDKVAC KDGDDFVQLP IPKLTPPDSE IVSHLTVRQT HTPYDYVVNG SVYFKYSPTT GQVTVIKKDE TFPKNMTVTQ DDNTSFIFNL NSEK

Formula weight

23938.43 Da

Source organism

Dictyostelium discoideum

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 82.9 %, Completeness (bb): 86.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.9 % (2068 of 2496)	89.1 % (1151 of 1292)	70.9 % (688 of 970)	97.9 % (229 of 234)
Backbone	86.1 % (1086 of 1262)	98.6 % (425 of 431)	73.4 % (461 of 628)	98.5 % (200 of 203)
Sidechain	82.2 % (1179 of 1434)	84.3 % (726 of 861)	78.2 % (424 of 542)	93.5 % (29 of 31)
Aromatic	12.7 % (31 of 244)	17.2 % (21 of 122)	5.9 % (7 of 119)	100.0 % (3 of 3)
Methyl	100.0 % (208 of 208)	100.0 % (104 of 104)	100.0 % (104 of 104)

1. DdCAD-1 monomer

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	DdCAD-1 monomer	[U-99% 13C; U-99% 15N]		0.7 ~ 0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.71 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.71 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.71 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2B1O, Strand ID: A Detail

Release date

2004-12-15

Citation 1

Letter to the Editor: 1H, 13C and 15N resonance assignments of Ca2+-free DdCAD-1: a Ca2+-dependent cell-cell adhesion molecule
Lin, Z., Huang, H., Siu, C., Yang, D.
J. Biomol. NMR (2004), 30, 375-376, PubMed , DOI:

Show more 2 citaions

Citation 2

Molecular cloning and characterization of DdCAD-1, a Ca2+-dependent cell-cell adhesion molecule, in Dictyostelium discoideum
Wong, E.F., Brar, S.K., Sesaki, H., Yang, C., Siu, C.H.
J. Biol. Chem. (1996), 271, 16399-16408, PubMed 8663243 , DOI: , Abstract

Citation 3

Disruption of the gene encoding the cell adhesion molecule DdCAD-1 leads to aberrant cell sorting and cell-type proportioning during Dictyostelium development
Wong, E., Yang, C., Wang, J., Fuller, D., Loomis, W.F., Siu, C.H.
Development (2002), 129, 3839-3850, PubMed 12135922 , DOI: , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P54657 released on 1996-10-01
Title CAD1_DICDI Entity Calcium-dependent cell adhesion molecule 1

Related entities 1. DdCAD-1 monomer, : 1 : 3 : 5 entities Detail

Interaction partners 1. DdCAD-1 monomer, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 CBCA(CO)NH