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BMRB: 6161

1UST

Full 1H, 13C and 15N Chemical Shift Assignments for the GI Domain of the yeast Linker Histone, Hho1p

Authors

Coles, P., Thomas, J.O., Stott, K., Stevens, T.J., Ali, T.

Assembly

Yeast linker histone globular domain I

Entity

1. Yeast linker histone globular domain I (polymer, Thiol state: not present), 93 monomers, 10256.67 Da Detail

KKEEASSKSY RELIIEGLTA LKERKGSSRP ALKKFIKENY PIVGSASNFD LYFNNAIKKG VEAGDFEQPK GPAGAVKLAK KKSPEVKKEK EVS

Formula weight

10256.67 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, BOND ANGLE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.8 %, Completeness (bb): 85.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.8 % (1029 of 1121)	98.7 % (590 of 598)	80.5 % (346 of 430)	100.0 % (93 of 93)
Backbone	85.0 % (466 of 548)	99.5 % (187 of 188)	70.2 % (191 of 272)	100.0 % (88 of 88)
Sidechain	98.5 % (649 of 659)	98.3 % (403 of 410)	98.8 % (241 of 244)	100.0 % (5 of 5)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. Yeast linker histone globular domain I

KKEEASSKSY RELIIEGLTA LKERKGSSRP ALKKFIKENY PIVGSASNFD LYFNNAIKKG VEAGDFEQPK GPAGAVKLAK KKSPEVKKEK EVS

Show sample condition

Sample

Temperature 288 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	Yeast linker histone globular domain I	[U-95% 13C; U-95% 15N]	2.7 mM
2	sodium phosphate		100 mM
3	DSS		10 mM
4	D2O		99.8 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.4048086
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: 0.11 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.4048086
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: 0.11 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.4048086
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1UST, Strand ID: A Detail

Release date

2004-06-29

Citation

Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p
Ali, T., Coles, P., Stevens, T.J., Stott, K., Thomas, J.O.
J. Mol. Biol. (2004), 338, 139-148, PubMed 15050829 , DOI 10.1016/j.jmb.2004.02.046 , Abstract

Related entities 1. Yeast linker histone globular domain I, : 1 : 2 : 1 : 45 entities Detail

Interaction partners 1. Yeast linker histone globular domain I, : 50 interactors Detail

More details for 50 interactors identified by 7 citations

Experiments performed 10 experiments Detail

1 NOESY-15N-HSQC