BMRB: 6162

Full 1H, 13C and 15N Chemical Shift Assignments for the GII Domain of the yeast Linker Histone, Hho1p

Authors

Coles, P., Thomas, J.O., Stott, K., Stevens, T.J., Ali, T.

Assembly

Yeast linker histone globular domain II

Entity

1. Yeast linker histone globular domain II (polymer, Thiol state: all free), 88 monomers, 9728.311 Da Detail

KASSPSSLTY KEMILKSMPQ LNDGKGSSRI VLKKYVKDTF SSKLKTSSNF DYLFNSAIKK CVENGELVQP KGPSGIIKLN KKKVKLST

Formula weight

9728.311 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, BOND ANGLE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.7 %, Completeness (bb): 83.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.7 % (918 of 1071)	91.5 % (520 of 568)	75.0 % (309 of 412)	97.8 % (89 of 91)
Backbone	83.8 % (436 of 520)	96.0 % (170 of 177)	71.0 % (184 of 259)	97.6 % (82 of 84)
Sidechain	88.8 % (563 of 634)	89.5 % (350 of 391)	87.3 % (206 of 236)	100.0 % (7 of 7)
Aromatic	7.4 % (4 of 54)	7.4 % (2 of 27)	7.4 % (2 of 27)
Methyl	95.5 % (84 of 88)	95.5 % (42 of 44)	95.5 % (42 of 44)

1. Yeast linker histone globular domain II

KASSPSSLTY KEMILKSMPQ LNDGKGSSRI VLKKYVKDTF SSKLKTSSNF DYLFNSAIKK CVENGELVQP KGPSGIIKLN KKKVKLST

Show sample condition

Sample

Temperature 288 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Yeast linker histone globular domain II	[U-95% 13C; U-95% 15N]		1.0 mM
2	sodium phosphate			250 mM
3	EDTA			1 mM
4	DTT			1 mM
5	DSS			10 mM
6	D2O			99.8 %

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LACS Plot; CA

Referencing offset: 0.2 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.4048086
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.4048086
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.4048086
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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Protein Blocks Logo

Calculated from 10 models in PDB: 1USS, Strand ID: A Detail

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Release date

2004-06-29

Citation

Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p
Ali, T., Coles, P., Stevens, T.J., Stott, K., Thomas, J.O.
J. Mol. Biol. (2004), 338, 139-148, PubMed 15050829 , DOI 10.1016/j.jmb.2004.02.046 , Abstract

Related entities 1. Yeast linker histone globular domain II, : 1 : 2 : 62 entities Detail

Interaction partners 1. Yeast linker histone globular domain II, : 50 interactors Detail

Experiments performed 9 experiments Detail