BMRB: 6165

Solution structure of the PSI domain from the Met receptor

Authors

Kozlov, G., Perreault, A., Schrag, J.D., Cygler, M., Gehring, K., Ekiel, I.

Assembly

Hepatocyte growth factor receptor

Entity

1. Hepatocyte growth factor receptor (polymer, Thiol state: all disulfide bound), 48 monomers, 5310.057 Da Detail

GSAMGCRHFQ SCSQCLSAPP FVQCGWCHDK CVRSEECLSG TWTQQICL

Formula weight

5310.057 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS24:SG
2	disulfide	sing	1:CYS12:SG	1:CYS47:SG
3	disulfide	sing	1:CYS15:SG	1:CYS31:SG
4	disulfide	sing	1:CYS27:SG	1:CYS37:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 80.4 %, Completeness (bb): 91.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	80.4 % (221 of 275)	80.4 % (221 of 275)
Backbone	91.8 % (90 of 98)	91.8 % (90 of 98)
Sidechain	74.0 % (131 of 177)	74.0 % (131 of 177)
Aromatic	65.4 % (17 of 26)	65.4 % (17 of 26)
Methyl	100.0 % (16 of 16)	100.0 % (16 of 16)

1. Hepatocyte growth factor receptor

GSAMGCRHFQ SCSQCLSAPP FVQCGWCHDK CVRSEECLSG TWTQQICL

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Hepatocyte growth factor receptor			1 mM
2	phosphate buffer			50 mM
3	NaCl			0.15 M
4	H2O			90 %
5	D2O			10 %

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Calculated from 20 models in PDB: 1SSL, Strand ID: A Detail

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Coupling constant

38 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 283 K, pH 6.0 Detail

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Pressure 1 atm, Temperature 283 K, pH 6.0

Experiment name 2D NOESY, DQF-COSY

List #1 coupling_constants_set_1, Field strength (¹H) 500 MHz

Release date

2004-04-01

Citation

Insights into function of PSI domains from structure of the Met receptor PSI domain
Kozlov, G., Perreault, A., Schrag, J.D., Park, M., Cygler, M., Gehring, K., Ekiel, I.
Biochem. Biophys. Res. Commun. (2004), 321, 234-240, PubMed 15358240 , DOI 10.1016/j.bbrc.2004.06.132 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Hepatocyte growth factor receptor, : 1 : 1 : 55 entities Detail

Interaction partners 1. Hepatocyte growth factor receptor, : 32 interactors Detail

Experiments performed 2 experiments Detail