BMRB: 6166

Solution structure of the second complement control protein (CCP) module of the GABA(B)R1a receptor, Pro-119 cis conformer

Authors

Blein, S.C., Uhrin, D., Smith, B.O., White, J.H., Barlow, P.N.

Assembly

Gamma-aminobutyric acid type B receptor

Entity

1. Gamma-aminobutyric acid type B receptor (polymer, Thiol state: all disulfide bound), 68 monomers, 7485.344 Da Detail

EAEFVRICSK SYLTLENGKV FLTGGDLPAL DGARVEFRCD PDFHLVGSSR SVCSQGQWST PKPHCQVN

Formula weight

7485.344 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS53:SG
2	disulfide	sing	1:CYS39:SG	1:CYS65:SG

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 82.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.2 % (692 of 767)	98.7 % (393 of 398)	76.9 % (230 of 299)	98.6 % (69 of 70)
Backbone	82.0 % (328 of 400)	97.8 % (135 of 138)	65.7 % (130 of 198)	98.4 % (63 of 64)
Sidechain	99.1 % (425 of 429)	99.2 % (258 of 260)	98.8 % (161 of 163)	100.0 % (6 of 6)
Aromatic	97.1 % (66 of 68)	97.1 % (33 of 34)	97.0 % (32 of 33)	100.0 % (1 of 1)
Methyl	98.4 % (63 of 64)	100.0 % (32 of 32)	96.9 % (31 of 32)

1. Gamma-aminobutyric acid type B receptor

EAEFVRICSK SYLTLENGKV FLTGGDLPAL DGARVEFRCD PDFHLVGSSR SVCSQGQWST PKPHCQVN

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 4

#	Name	Isotope labeling	Type	Concentration
1	Gamma-aminobutyric acid type B receptor	[U-15N; U-13C]		1 mM
2	deuterated sodium acetate			20 mM
3	D2O			10 %
4	H2O			90 %

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LACS Plot; CA

Referencing offset: -0.45 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.766 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.45 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.766 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.766 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 24 models in PDB: 1SS2, Strand ID: A Detail

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Coupling constant

23 J values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 310 K, pH 4 Detail

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Pressure 1 atm, Temperature 310 K, pH 4

List #1 coupling_constants_set_1, Field strength (¹H) 800 MHz

Release date

2004-09-06

Citation

Structural analysis of the complement control protein (CCP) modules of GABA(B) receptor 1a: only one of the two CCP modules is compactly folded
Blein, S.C., Ginham, R., Uhrin, D., Smith, B.O., Soares, D.C., Veltel, S., McIlhinney, R.J., White, J.H., Barlow, P.N.
J. Biol. Chem. (2004), 279, 48292-48306, PubMed 15304491 , DOI 10.1074/jbc.M406540200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. Gamma-aminobutyric acid type B receptor, : 1 : 1 : 2 : 61 entities Detail

Interaction partners 1. Gamma-aminobutyric acid type B receptor, : 3 interactors Detail

Experiments performed 3 experiments Detail