BMRBj - BMREntryMaster

Found 1 Document (1.85 seconds)

BMRB: 6167

1SNL

Structural studies on the Ca-binding domain of human nucleobindin (CALNUC)

Authors

de Alba, E., Tjandra, N.

Assembly

Nucleobindin

Entity

1. Nucleobindin (polymer, Thiol state: not present), 99 monomers, 11990.20 Da Detail

LKEVWEELDG LDPNRFNPKT FFILHDINSD GVLDEQELEA LFTKELEKVY DPKNEEDDMR EMEEERLRMR EHVMKNVDTN QDRLVTLEEF LASTQRKEF

2. CA (non-polymer), 40.078 × 2 Da

Total weight

12070.356 Da

Max. entity weight

11990.2 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 86.7 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.7 % (1054 of 1215)	82.2 % (528 of 642)	91.6 % (428 of 467)	92.5 % (98 of 106)
Backbone	93.2 % (548 of 588)	94.4 % (186 of 197)	92.9 % (274 of 295)	91.7 % (88 of 96)
Sidechain	82.9 % (600 of 724)	76.9 % (342 of 445)	92.2 % (248 of 269)	100.0 % (10 of 10)
Aromatic	77.3 % (68 of 88)	79.5 % (35 of 44)	74.4 % (32 of 43)	100.0 % (1 of 1)
Methyl	94.7 % (89 of 94)	95.7 % (45 of 47)	93.6 % (44 of 47)

1. Nucleobindin

LKEVWEELDG LDPNRFNPKT FFILHDINSD GVLDEQELEA LFTKELEKVY DPKNEEDDMR EMEEERLRMR EHVMKNVDTN QDRLVTLEEF LASTQRKEF

Show sample condition

Sample

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.21 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.21 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.28 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1SNL, Strand ID: A Detail

Release date

2004-06-24

Citation

Structural studies on the Ca-binding domain of human nucleobindin (CALNUC)
de Alba, E., Tjandra, N.
Biochemistry

Related entities 1. Nucleobindin, : 1 : 6 : 1 : 1 : 41 entities Detail

Interaction partners 1. Nucleobindin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 15N-HSQC

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

2 3D-15N separated-NOESY

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

3 4D-15N

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

4 13C separated NOESY

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

5 HNCO

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

6 CBCACONH

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

7 HNCACB

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

8 HCCONH

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

9 CCONH

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

10 4D-13C,13C separated NOESY

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

11 HCCH-TOCSY

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

12 HCACONH

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

13 13C-HSQC JMOD

Instrument

#1 Model Bruker AVANCE (800 MHz)

#2 Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	Nucleobindin	[U-15N]	1 mM
2	CaCl2		5 mM
3	NaN3		0.1 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
6	Nucleobindin	[U-15N; U-13C]	1 mM
7	CaCl2		5 mM
8	NaN3		0.1 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
11	Nucleobindin	[U-15N; U-13C]	1 mM
12	CaCl2		5 mM
13	NaN3		0.1 mM
14	D2O		100 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
15	Nucleobindin	[U-15N; U-13C]	0.7 mM
16	CaCl2		5 mM
17	NaN3		0.1 mM
18	H2O		90 %
19	D2O		10 %

nullKeywords EF-HAND, CALCIUM-BINDING, NMR

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.85 seconds)

BMRB: 6167

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Nucleobindin, : 1 : 6 : 1 : 1 : 41 entities Detail

Interaction partners 1. Nucleobindin, : 1 interactors Detail

Experiments performed 13 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1