BMRB: 6172

Solution structure of a cyanobacterial copper metallochaperone, ScAtx1

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Su, X.C., Borrelly, G., Robinson, N.J.

Assembly

apo ScAtx1

Entity

1. apo ScAtx1 (polymer, Thiol state: all free), 64 monomers, 6685.471 Da Detail

MTIQLTVPTI ACEACAEAVT KAVQNEDAQA TVQVDLTSKK VTITSALGEE QLRTAIASAG HEVE

Formula weight

6685.471 Da

Source organism

Synechocystis sp. PCC 6803

Exptl. method

NMR

Refine. method

distance geometry,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 91.0 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	91.0 % (373 of 410)	90.0 % (307 of 341)	95.7 % (66 of 69)
Backbone	95.8 % (184 of 192)	96.1 % (124 of 129)	95.2 % (60 of 63)
Sidechain	86.7 % (189 of 218)	86.3 % (183 of 212)	100.0 % (6 of 6)
Aromatic	100.0 % (2 of 2)	100.0 % (2 of 2)
Methyl	100.0 % (50 of 50)	100.0 % (50 of 50)

1. apoScAtx1

MTIQLTVPTI ACEACAEAVT KAVQNEDAQA TVQVDLTSKK VTITSALGEE QLRTAIASAG HEVE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	apoScAtx1	[U-15N]		0.7 mM
2	phosphate			50 mM
3	H2O			90 %
4	D2O			10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1SB6, Strand ID: A Detail

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Release date

2004-06-28

Citation

Solution structures of a cyanobacterial metallochaperone: insight into an atypical copper-binding motif
Banci, L., Bertini, I., Ciofi-Baffoni, S., Su, X.C., Borrelly, G.P., Robinson, N.J.
J. Biol. Chem. (2004), 279, 27502-27510, PubMed 15075318 , DOI 10.1074/jbc.M402005200 , Abstract

Related entities 1. apo ScAtx1, : 1 : 4 : 2 : 1 : 99 entities Detail

Experiments performed 4 experiments Detail