BMRB: 6175

1H, 13C, and 15N assignments for the first TSR domain of F-spondin

Authors

Tossavainen, H.

Assembly

F-spondin TSR domain 1

Entity

1. F-spondin TSR domain 1 (polymer, Thiol state: all disulfide bound), 61 monomers, 6707.456 Da Detail

GSETCIYSNW SPWSACSSST CEKGKRMRQR MLKAQLDLSV PCPDTQDFQP CMGPGCSDED G

Formula weight

6707.456 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS42:SG
2	disulfide	sing	1:CYS16:SG	1:CYS51:SG
3	disulfide	sing	1:CYS21:SG	1:CYS56:SG

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 97.5 %, Completeness (bb): 97.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.5 % (650 of 667)	98.3 % (346 of 352)	96.4 % (243 of 252)	96.8 % (61 of 63)
Backbone	97.5 % (347 of 356)	96.7 % (118 of 122)	98.3 % (175 of 178)	96.4 % (54 of 56)
Sidechain	97.8 % (359 of 367)	99.1 % (228 of 230)	95.4 % (124 of 130)	100.0 % (7 of 7)
Aromatic	90.5 % (38 of 42)	100.0 % (21 of 21)	78.9 % (15 of 19)	100.0 % (2 of 2)
Methyl	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)

1. thrombospondin type-1 repeat

GSETCIYSNW SPWSACSSST CEKGKRMRQR MLKAQLDLSV PCPDTQDFQP CMGPGCSDED G

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Sample

Temperature 283 (±0.5) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	thrombospondin type-1 repeat	[U-13C; U-15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.908 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.908 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.908 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.7 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
1H	water	protons	4.908 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1SZL, Strand ID: A Detail

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Release date

2004-04-11

Citation

Solution structures of the first and fourth TSR domains of F-spondin
Paakkonen, K., Tossavainen, H., Permi, P., Rakkolainen, H., Rauvala, H., Raulo, E., Kilpelainen, I., Guntert, P.
Proteins (2006), 64, 665-672, PubMed 16736493 , DOI 10.1002/prot.21030 , Abstract

Related entities 1. F-spondin TSR domain 1, : 1 : 5 : 112 entities Detail

Interaction partners 1. F-spondin TSR domain 1, : 7 interactors Detail

Experiments performed 10 experiments Detail