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BMRB: 6178

1SJQ

N-terminal Domain of the Polypyrimidine Tract Binding Protein Contains a New RRM Variant That Contributes to High-Affinity RNA Binding

Authors

Simpson, P.J., Monie, T.P., Szendroi, A., Davydova, N., Tyzack, J.K., Conte, M.R., Read, C.M., Cary, P.D., Svergun, D.I., Konarev, P.V., Petoukhov, M.V., Curry, S., Matthews, S.J.

Assembly

Polypyrimidine tract-binding protein 1 domain 1

Entity

1. Polypyrimidine tract-binding protein 1 domain 1 (polymer, Thiol state: not present), 105 monomers, 11726.25 Da Detail

MRGSHHHHHH GSGVPSRVIH IRKLPIDVTE GEVISLGLPF GKVTNLLMLK GKNQAFIEMN TEEAANTMVN YYTSVTPVLR GQPIYIQFSN HKELKTDSSP NQARA

Formula weight

11726.25 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Hybrid distance geometry/simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.5 %, Completeness: 84.0 %, Completeness (bb): 85.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.0 % (1027 of 1222)	82.7 % (526 of 636)	85.9 % (409 of 476)	83.6 % (92 of 110)
Backbone	85.3 % (527 of 618)	83.5 % (177 of 212)	87.0 % (267 of 307)	83.8 % (83 of 99)
Sidechain	83.5 % (585 of 701)	82.3 % (349 of 424)	85.3 % (227 of 266)	81.8 % (9 of 11)
Aromatic	65.1 % (56 of 86)	65.1 % (28 of 43)	65.1 % (28 of 43)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. polypyrimidine tract binding protein 1 domain 1

MRGSHHHHHH GSGVPSRVIH IRKLPIDVTE GEVISLGLPF GKVTNLLMLK GKNQAFIEMN TEEAANTMVN YYTSVTPVLR GQPIYIQFSN HKELKTDSSP NQARA

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±2) K, pH 6.5 (±0.2)

#	Name	Concentration
1	polypyrimidine tract binding protein 1 domain 1	1 mM
2	Na phosphate	50 mM
3	NaCl	100 mM
4	sodium azide	2 mM
5	DTT	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.54 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.54 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291
¹³C	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.37 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 1SJQ, Strand ID: A Detail

Release date

2004-11-07

Citation

Structure and RNA interactions of the N-terminal RRM domains of PTB
Simpson, P.J., Monie, T.P., Szendroi, A., Davydova, N., Tyzack, J.K., Conte, M.R., Read, C.M., Cary, P.D., Svergun, D.I., Konarev, P.V., Curry, S., Matthews, S.J.
Structure (2004), 12, 1631-1643, PubMed 15341728 , DOI 10.1016/j.str.2004.07.008 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 6177 released on 2004-11-07
    Title N-terminal Domain of the Polypyrimidine Tract Binding Protein Contains a New RRM Variant That Contributes to High-Affinity RNA Binding
  Swiss-Prot: P26599 released on 1992-08-01
    Title PTBP1_HUMAN Entity Polypyrimidine tract-binding protein 1

Related entities 1. Polypyrimidine tract-binding protein 1 domain 1, : 1 : 1 : 93 entities Detail

Interaction partners 1. Polypyrimidine tract-binding protein 1 domain 1, : 96 interactors Detail

More details for 96 interactors identified by 38 citations

Experiments performed 8 experiments Detail

1 HSQC