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Found 1 Document (1.622 seconds)

BMRB: 6179

1UVF

Solution Structure of the structured part of the 15th domain of LEKTI

Authors

Vitzithum, K., Roesch, P., Marx, U.C.

Assembly

LEKTI Domain 15 short

Entity

1. LEKTI Domain 15 short (polymer, Thiol state: all disulfide bound), 61 monomers, 6928.866 Da Detail

GPDSEMCKDY RVLPRIGYLC PKDLKPVCGD DGQTYNNPCM LCHENLIRQT NTHIRSTGKC E

Formula weight

6928.866 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS42:SG
2	disulfide	sing	1:CYS20:SG	1:CYS39:SG
3	disulfide	sing	1:CYS28:SG	1:CYS60:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 84.7 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	84.7 % (364 of 430)	84.8 % (312 of 368)	83.9 % (52 of 62)
Backbone	93.8 % (167 of 178)	96.7 % (118 of 122)	87.5 % (49 of 56)
Sidechain	78.2 % (197 of 252)	78.9 % (194 of 246)	50.0 % (3 of 6)
Aromatic	75.0 % (12 of 16)	75.0 % (12 of 16)
Methyl	95.8 % (23 of 24)	95.8 % (23 of 24)

1. LEKTI Domain 15 short

GPDSEMCKDY RVLPRIGYLC PKDLKPVCGD DGQTYNNPCM LCHENLIRQT NTHIRSTGKC E

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Protein Blocks Logo

Calculated from 31 models in PDB: 1UVF, Strand ID: A Detail

Release date

2004-04-14

Citation

The solution structure of a chimeric LEKTI domain reveals a chameleon sequence
Tidow, H., Lauber, T., Vitzithum, K., Sommerhoff, C.P., Roesch, P., Marx, U.C.
Biochemistry (2004), 43, 11238-11247, PubMed 15366933 , DOI 10.1021/bi0492399 , Abstract

Related entities 1. LEKTI Domain 15 short, : 1 : 1 : 2 : 74 entities Detail

Experiments performed 8 experiments Detail

1 2D-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

2 2D-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

3 2D-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

4 1H,15N-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

5 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

6 3D-1H,1H,15N-TOCSY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

7 3D-1H,1H,15N-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

8 3D-1H,15N,15N-HMQC-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15 short	[U-15N]		0.7 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.6 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15 short			1.6 mM

nullKeywords Kazal, LEKTI, Serine Proteinase Inhibitor, Trypsin Inhibitor

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Found 1 Document (1.622 seconds)

BMRB: 6179

Sample

Related entities 1. LEKTI Domain 15 short, : 1 : 1 : 2 : 74 entities Detail

Experiments performed 8 experiments Detail

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