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Found 1 Document (2.022 seconds)

BMRB: 6180

1UVG

Solution structure of the 15th domain of LEKTI

Authors

Vitzithum, K., Roesch, P., Marx, U.C.

Assembly

LEKTI Domain 15

Entity

1. LEKTI Domain 15 (polymer, Thiol state: all disulfide bound), 78 monomers, 8575.550 Da Detail

GPDSEMCKDY RVLPRIGYLC PKDLKPVCGD DGQTYNNPCM LCHENLIRQT NTHIRSTGKC EESSTPGTTA ASMPPSDE

Formula weight

8575.55 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS42:SG
2	disulfide	sing	1:CYS20:SG	1:CYS39:SG
3	disulfide	sing	1:CYS28:SG	1:CYS60:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 85.0 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	85.0 % (446 of 525)	82.6 % (371 of 449)	98.7 % (75 of 76)
Backbone	97.3 % (218 of 224)	96.8 % (149 of 154)	98.6 % (69 of 70)
Sidechain	75.7 % (228 of 301)	75.3 % (222 of 295)	100.0 % (6 of 6)
Aromatic	75.0 % (12 of 16)	75.0 % (12 of 16)
Methyl	100.0 % (29 of 29)	100.0 % (29 of 29)

1. LEKTI Domain 15

GPDSEMCKDY RVLPRIGYLC PKDLKPVCGD DGQTYNNPCM LCHENLIRQT NTHIRSTGKC EESSTPGTTA ASMPPSDE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Protein Blocks Logo

Calculated from 31 models in PDB: 1UVG, Strand ID: A Detail

Release date

2005-05-16

Citation

The solution structure of a chimeric LEKTI domain reveals a chameleon sequence
Tidow, H., Lauber, T., Vitzithum, K., Sommerhoff, C.P., Roesch, P., Marx, U.C.
Biochemistry (2004), 43, 11238-11247, PubMed 15366933 , DOI 10.1021/bi0492399 , Abstract

Related entities 1. LEKTI Domain 15, : 1 : 2 : 20 entities Detail

Experiments performed 8 experiments Detail

1 2D-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

2 2D-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

3 2D-COESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

4 1H,15N-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

5 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

6 3D-1H,1H,15-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

7 3D-1H,1H,15N-TOCSY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

8 3D-1H,15N,15N-HMQC-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	LEKTI Domain 15	[U-15N]		1.6 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	LEKTI Domain 15			2.3 mM

nullKeywords Serine Proteinase Inhibitor, Trypsin Inhibitor, Kazal, LEKTI

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Found 1 Document (2.022 seconds)

BMRB: 6180

Sample

Related entities 1. LEKTI Domain 15, : 1 : 2 : 20 entities Detail

Experiments performed 8 experiments Detail

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