BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.0 % (626 of 711)	95.7 % (352 of 368)	76.2 % (211 of 277)	95.5 % (63 of 66)
Backbone	81.6 % (297 of 364)	96.0 % (119 of 124)	67.2 % (121 of 180)	95.0 % (57 of 60)
Sidechain	95.1 % (385 of 405)	95.5 % (233 of 244)	94.2 % (146 of 155)	100.0 % (6 of 6)
Aromatic	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (28 of 28)	100.0 % (1 of 1)
Methyl	91.9 % (68 of 74)	94.6 % (35 of 37)	89.2 % (33 of 37)

1. Regulatory protein cro

MYKKDVIDHF GTQRAVAKAL GISDAAVSQW KEVIPEKDAY RLEIVTAGAL KYQENAYRQA A

Show sample condition

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 1RZS, Strand ID: A Detail

Release date

2004-06-03

Citation

Secondary structure switching in Cro protein evolution
Newlove, T., Konieczka, J.H., Cordes, M.H.
Structure (2004), 12, 569-581, PubMed 15062080 , DOI 10.1016/j.str.2004.02.024 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P09964 released on 1989-07-01
Title RCRO_BPP22 Entity Regulatory protein cro

Related entities 1. Regulatory protein cro, : 1 : 1 : 9 entities Detail

Experiments performed 6 experiments Detail

1 2D NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
13	Regulatory protein cro	[U-15N]	3.5 mM
14	sodium phosphate buffer		50 mM
15	D2O		100 %

2 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
13	Regulatory protein cro	[U-15N]	3.5 mM
14	sodium phosphate buffer		50 mM
15	D2O		100 %

3 HNHB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
13	Regulatory protein cro	[U-15N]	3.5 mM
14	sodium phosphate buffer		50 mM
15	D2O		100 %

4 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
13	Regulatory protein cro	[U-15N]	3.5 mM
14	sodium phosphate buffer		50 mM
15	D2O		100 %

5 3D 13C-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
13	Regulatory protein cro	[U-15N]	3.5 mM
14	sodium phosphate buffer		50 mM
15	D2O		100 %

6 hydrogen exchange

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.4

#	Name	Concentration
1	Regulatory protein cro	6 mM
2	sodium phosphate buffer	50 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
5	Regulatory protein cro	[U-15N]	3.5 mM
6	sodium phosphate buffer		50 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
9	Regulatory protein cro	[U-13C; U-15N]	2.75 mM
10	sodium phosphate buffer		50 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, pH 6.4

#	Name	Isotope labeling	Concentration
13	Regulatory protein cro	[U-15N]	3.5 mM
14	sodium phosphate buffer		50 mM
15	D2O		100 %

nullKeywords HELIX-TURN-HELIX, DNA-BINDING PROTEIN, STRUCTURAL EVOLUTION

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BMRB: 6185

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Regulatory protein cro, : 1 : 1 : 9 entities Detail

Experiments performed 6 experiments Detail

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