BMRBj - BMREntryMaster

Found 1 Document (1.576 seconds)

BMRB: 6187

1SF0

BACKBONE SOLUTION STRUCTURE OF MIXED ALPHA/BETA PROTEIN PF1061

Authors

Prestegard, J.H., Mayer, K.L., Valafar, H.

Assembly

hypothetical protein PF1061

Entity

1. hypothetical protein PF1061 (polymer, Thiol state: not present), 77 monomers, 8592.735 Da Detail

AHHHHHHGSK MIKVKVIGRN IEKEIEWREG MKVRDILRAV GFNTESAIAK VNGKVVLEDD EVKDGDFVEV IPVVSGG

Formula weight

8592.735 Da

Source organism

Pyrococcus furiosus

Exptl. method

Refine. method

RDC DIRECTED FRAGMENT ASSEMBLY

Data set

assigned_chemical_shifts, coupling_constants, RDCs

Chem. Shift Complete

Sequence coverage: 88.3 %, Completeness: 31.8 %, Completeness (bb): 60.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.8 % (283 of 890)	31.2 % (144 of 462)	20.4 % (71 of 348)	85.0 % (68 of 80)
Backbone	60.4 % (278 of 460)	87.0 % (140 of 161)	31.4 % (70 of 223)	89.5 % (68 of 76)
Sidechain	1.2 % (6 of 499)	1.3 % (4 of 301)	1.0 % (2 of 194)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 56)	0.0 % (0 of 28)	0.0 % (0 of 27)	0.0 % (0 of 1)
Methyl	0.0 % (0 of 94)	0.0 % (0 of 47)	0.0 % (0 of 47)

1. hypothetical protein PF1061

AHHHHHHGSK MIKVKVIGRN IEKEIEWREG MKVRDILRAV GFNTESAIAK VNGKVVLEDD EVKDGDFVEV IPVVSGG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
19	hypothetical protein PF1061	[U-15N]		1 mM
20	PHOSPHATE BUFFER			50 mM
21	KCL			200 mM
22	H2O			90 %
23	D2O			10 %

Protein Blocks Logo

Calculated from 1 models in PDB: 1SF0, Strand ID: A Detail

Coupling constant

59 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 5.5 Detail

RDC

394 RDC values in 6 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 5.5 Detail

Release date

2004-06-24

Citation

Backbone solution structures of proteins using residual dipolar couplings: application to a novel structural genomics target
Valafar, H., Mayer, K.L., Bougault, C.M., LeBlond, P.D., Jenney, F.E., Brereton, P.S., Adams, M.W.W., Prestegard, J.H.
J. Struct. Funct. Genomics (2004), 5, 241-254, PubMed 15704012 , DOI 10.1007/s10969-005-4899-5 , Abstract

Related entities 1. hypothetical protein PF1061, : 1 : 1 : 1 : 7 entities Detail

Experiments performed 10 experiments Detail

1 3D 15N-SEPARATED NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
19	hypothetical protein PF1061	[U-15N]		1 mM
20	PHOSPHATE BUFFER			50 mM
21	KCL			200 mM
22	H2O			90 %
23	D2O			10 %

2 3D 15N-SEPARATED TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
19	hypothetical protein PF1061	[U-15N]		1 mM
20	PHOSPHATE BUFFER			50 mM
21	KCL			200 mM
22	H2O			90 %
23	D2O			10 %

3 SOFT HNCA-E.COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	hypothetical protein PF1061	[U-15N; 16%-13C]		1 mM
2	PHOSPHATE BUFFER			50 mM
3	KCL			200 mM
4	H2O			90 %
5	D2O			10 %

4 MODIFIED HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	hypothetical protein PF1061	[U-15N; 16%-13C]		1 mM
2	PHOSPHATE BUFFER			50 mM
3	KCL			200 mM
4	H2O			90 %
5	D2O			10 %

5 15N COUPLED HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	hypothetical protein PF1061	[U-15N; 16%-13C]		1 mM
2	PHOSPHATE BUFFER			50 mM
3	KCL			200 mM
4	H2O			90 %
5	D2O			10 %

6 SOFT HNCA-E.COSY

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Pressure 1 atm, Temperature 300 K, pH 6, Details the C12E5 conentration is 4%

#	Name	Isotope labeling	Type	Concentration
6	hypothetical protein PF1061	[U-15N; 16%-13C]		0.5 mM
7	PHOSPHATE BUFFER			50 mM
8	C12E5:HEXANOL 0.98:1
9	KCL			100 mM
10	H2O			90 %
11	D2O			10 %

7 MODIFIED HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Pressure 1 atm, Temperature 300 K, pH 6, Details the C12E5 conentration is 4%

#	Name	Isotope labeling	Type	Concentration
6	hypothetical protein PF1061	[U-15N; 16%-13C]		0.5 mM
7	PHOSPHATE BUFFER			50 mM
8	C12E5:HEXANOL 0.98:1
9	KCL			100 mM
10	H2O			90 %
11	D2O			10 %

8 15N COUPLED HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Pressure 1 atm, Temperature 300 K, pH 6, Details the C12E5 conentration is 4%

#	Name	Isotope labeling	Type	Concentration
6	hypothetical protein PF1061	[U-15N; 16%-13C]		0.5 mM
7	PHOSPHATE BUFFER			50 mM
8	C12E5:HEXANOL 0.98:1
9	KCL			100 mM
10	H2O			90 %
11	D2O			10 %

9 15N COUPLED HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Pressure 1 atm, Temperature 293 K, pH 6, Details the C12E5 conentration is 4%

#	Name	Isotope labeling	Type	Concentration
12	hypothetical protein PF1061	[U-15N; 16%-13C]		0.5 mM
13	PHOSPHATE BUFFER			50 mM
14	PEG:CTAB 27:1
15	C12E5:HEXANOL 0.87:1
16	KCL			100 mM
17	H2O			90 %
18	D2O			10 %

10 SOFT HNCA-E.COSY

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Pressure 1 atm, Temperature 293 K, pH 6, Details the C12E5 conentration is 4%

#	Name	Isotope labeling	Type	Concentration
12	hypothetical protein PF1061	[U-15N; 16%-13C]		0.5 mM
13	PHOSPHATE BUFFER			50 mM
14	PEG:CTAB 27:1
15	C12E5:HEXANOL 0.87:1
16	KCL			100 mM
17	H2O			90 %
18	D2O			10 %

nullKeywords NMR, RESIDUAL DIPOLAR COUPLINGS, STRUCTURAL GENOMICS