BMRB: 6189

Solution structure of SEP domain from human p47

Authors

Soukenik, M., Leidert, M., Sievert, V., Buessow, K., Leitner, D., Labudde, D., Ball, L., Oschkinat, H.

Assembly

NSFL1 cofactor p47

Entity

1. NSFL1 cofactor p47 (polymer, Thiol state: not present), 102 monomers, 11349.43 Da Detail

GSEKRQHSSQ DVHVVLKLWK SGFSLDNGEL RSYQDPSNAQ FLESIRRGEV PAELRRLAHG GQVNLDMEDH RDEDFVKPKG AFKAFTGEGQ KLGSTAPQVL ST

Formula weight

11349.43 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.6 %, Completeness (bb): 84.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.6 % (958 of 1174)	84.8 % (523 of 617)	73.4 % (329 of 448)	97.2 % (106 of 109)
Backbone	84.4 % (510 of 604)	97.1 % (204 of 210)	70.6 % (209 of 296)	99.0 % (97 of 98)
Sidechain	81.4 % (539 of 662)	78.4 % (319 of 407)	86.5 % (211 of 244)	81.8 % (9 of 11)
Aromatic	41.9 % (36 of 86)	41.9 % (18 of 43)	40.5 % (17 of 42)	100.0 % (1 of 1)
Methyl	98.9 % (89 of 90)	100.0 % (45 of 45)	97.8 % (44 of 45)

1. NSFL1 cofactor p47

GSEKRQHSSQ DVHVVLKLWK SGFSLDNGEL RSYQDPSNAQ FLESIRRGEV PAELRRLAHG GQVNLDMEDH RDEDFVKPKG AFKAFTGEGQ KLGSTAPQVL ST

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 5.6

#	Name	Isotope labeling	Type	Concentration
1	NSFL1 cofactor p47	[U-15N]		2 mM
2	phosphate buffer			20 mM
3	sodium chloride			100 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.101329
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.101329
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.101329
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1SS6, Strand ID: A Detail

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Release date

2004-11-07

Citation

The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L
Soukenik, M., Diehl, A., Leidert, M., Sievert, V., Bussow, K., Leitner, D., Labudde, D., Ball, L.J., Lechner, A., Nagler, D.K., Oschkinat, H.
FEBS Lett. (2004), 576, 358-362, PubMed 15498563 , DOI 10.1016/j.febslet.2004.09.037 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. NSFL1 cofactor p47, : 1 : 2 : 42 entities Detail

Interaction partners 1. NSFL1 cofactor p47, : 28 interactors Detail

Experiments performed 3 experiments Detail