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Found 1 Document (4.873 seconds)

BMRB: 6190

1SO9

Solution Structure of apoCox11

Authors

Banci, L., Bertini, I., Cantini, F., Ciofi-Baffoni, S., Gonnelli, L., Mangani, S.

Assembly

Cytochrome C oxidase assembly protein ctaG

Entity

1. Cytochrome C oxidase assembly protein ctaG (polymer, Thiol state: all free), 164 monomers, 18371.93 Da Detail

MVPLYDMFCR VTGYNGTTQR VEQASDLILD EKIKVTFDAN VAAGLPWEFV PVQRDIDVRI GETVQIMYRA KNLASTPTTG QATFNVTPMA AGAYFNKVQC FCFTETTLEP GEEMEMPVVF FVDPEIVKPV ETQGIKTLTL SYTFYPREPS KPVAQVKAKA ENKL

Formula weight

18371.93 Da

Source organism

Sinorhizobium meliloti

Exptl. method

NMR

Refine. method

torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.0 %, Completeness: 66.7 %, Completeness (bb): 69.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.7 % (1284 of 1925)	76.2 % (758 of 995)	50.5 % (385 of 763)	84.4 % (141 of 167)
Backbone	69.6 % (668 of 960)	86.1 % (279 of 324)	54.1 % (262 of 484)	83.6 % (127 of 152)
Sidechain	65.7 % (737 of 1121)	71.4 % (479 of 671)	56.1 % (244 of 435)	93.3 % (14 of 15)
Aromatic	23.7 % (38 of 160)	46.3 % (37 of 80)	0.0 % (0 of 79)	100.0 % (1 of 1)
Methyl	74.5 % (146 of 196)	82.7 % (81 of 98)	66.3 % (65 of 98)

1. Cytochrome C oxidase assembly protein ctaG

MVPLYDMFCR VTGYNGTTQR VEQASDLILD EKIKVTFDAN VAAGLPWEFV PVQRDIDVRI GETVQIMYRA KNLASTPTTG QATFNVTPMA AGAYFNKVQC FCFTETTLEP GEEMEMPVVF FVDPEIVKPV ETQGIKTLTL SYTFYPREPS KPVAQVKAKA ENKL

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 1.21 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 1.21 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1SP0, Strand ID: A Detail

Release date

2004-06-29

Citation

Solution structure of Cox11, a novel type of beta-immunoglobulin-like fold involved in CuB site formation of cytochrome c oxidase
Banci, L., Bertini, I., Cantini, F., Ciofi-Baffoni, S., Gonnelli, L., Mangani, S.
J. Biol. Chem. (2004), 279, 34833-34839, PubMed 15181013 , DOI 10.1074/jbc.M403655200 , Abstract

Related entities 1. Cytochrome C oxidase assembly protein ctaG, : 1 : 1 : 1 : 65 entities Detail

Experiments performed 10 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

2 2D TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

3 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

4 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

5 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

6 13C (H)CCH-TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

7 CBCA(CO)NH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

8 CBCANH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

9 HNCO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

10 HN(CA)CO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Cytochrome C oxidase assembly protein ctaG	[U-15N]	1.5 mM
2	DTT		5 mM
3	phosphate buffer		20 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	1 mM
7	DTT		5 mM
8	phosphate buffer		20 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Cytochrome C oxidase assembly protein ctaG	[U-95% 13C; U-98% 15N]	2 mM
12	DTT		5 mM
13	phosphate buffer		20 mM
14	H2O		90 %
15	D2O		10 %

nullKeywords immunoglobulin-like fold, copper protein, cytochrome c oxidase assembly

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Found 1 Document (4.873 seconds)

BMRB: 6190

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Cytochrome C oxidase assembly protein ctaG, : 1 : 1 : 1 : 65 entities Detail

Experiments performed 10 experiments Detail

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