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Found 1 Document (3.366 seconds)

BMRB: 6191

1SX0

Structure of the C-Terminal Domain of the SecA ATPase

Authors

Dempsey, B.R., Wrona, M., Moulin, J.M., Gloor, G.B., Jalilehvand, F., Lajoie, G., Shaw, G.S., Shilton, B.H.

Assembly

SecA

Entity

1. SecA (polymer, Thiol state: all other bound), 22 monomers, 2418.779 Da Detail

KVGRNDPCPC GSGKKYKQCH GR

2. ZN (non-polymer), 65.409 Da

Total weight

2484.188 Da

Max. entity weight

2418.779 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS10:SG	2:ZN1:ZN
3	na	sing	1:CYS19:SG	2:ZN1:ZN
4	na	sing	1:HIS20:ND1	2:ZN1:ZN

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.0 %, Completeness (bb): 64.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	64.0 % (146 of 228)	84.7 % (116 of 137)	33.0 % (30 of 91)
Backbone	64.8 % (70 of 108)	100.0 % (46 of 46)	38.7 % (24 of 62)
Sidechain	56.5 % (78 of 138)	76.9 % (70 of 91)	17.0 % (8 of 47)
Aromatic	33.3 % (4 of 12)	66.7 % (4 of 6)	0.0 % (0 of 6)
Methyl	25.0 % (1 of 4)	50.0 % (1 of 2)	0.0 % (0 of 2)

1. SecA

KVGRNDPCPC GSGKKYKQCH GR

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	SecA	1.7 mM
2	deuterated Pipes buffer	20 mM
3	NaCl	50 mM
4	TCEP	0.5 mM
5	ZnCl2	3.4 mM
6	H2O	90 %
7	D2O	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1SX1, Strand ID: A Detail

Release date

2004-09-06

Citation

Solution NMR structure and X-ray absorption analysis of the C-terminal zinc-binding domain of the SecA ATPase
Dempsey, B.R., Wrona, M., Moulin, J.M., Gloor, G.B., Jalilehvand, F., Lajoie, G., Shaw, G.S., Shilton, B.H.
Biochemistry (2004), 43, 9361-9371, PubMed 15260479 , DOI 10.1021/bi0493057 , Abstract

Related entities 1. SecA, : 1 : 22 : 67 : 3 : 30 entities Detail

Interaction partners 1. SecA, : 94 interactors Detail

More details for 94 interactors identified by 10 citations

Experiments performed 2 experiments Detail

#	Name	Concentration
8	SecA	3.5 mM
9	deuterated Pipes buffer	20 mM
10	NaCl	50 mM
11	NaN3	5 mM
12	TCEP	0.5 mM
13	ZnCl2	7 mM
14	H2O	90 %
15	D2O	10 %

#	Name	Concentration
16	SecA	1.7 mM
17	deuterated Pipes buffer	20 mM
18	NaCl	50 mM
19	NaN3	5 mM
20	TCEP	0.5 mM
21	ZnCl2	3.4 mM
22	D2O	100 %

#	Name	Concentration
23	SecA	3.5 mM
24	deuterated Pipes buffer	20 mM
25	NaCl	50 mM
26	NaN3	5 mM
27	TCEP	0.5 mM
28	ZnCl2	7 mM
29	D2O	100 %

#	Name	Concentration
8	SecA	3.5 mM
9	deuterated Pipes buffer	20 mM
10	NaCl	50 mM
11	NaN3	5 mM
12	TCEP	0.5 mM
13	ZnCl2	7 mM
14	H2O	90 %
15	D2O	10 %

#	Name	Concentration
16	SecA	1.7 mM
17	deuterated Pipes buffer	20 mM
18	NaCl	50 mM
19	NaN3	5 mM
20	TCEP	0.5 mM
21	ZnCl2	3.4 mM
22	D2O	100 %

#	Name	Concentration
23	SecA	3.5 mM
24	deuterated Pipes buffer	20 mM
25	NaCl	50 mM
26	NaN3	5 mM
27	TCEP	0.5 mM
28	ZnCl2	7 mM
29	D2O	100 %

nullKeywords metal ion, no secondary structure, structural zinc coordination, tetrahedral coordination, zinc

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Found 1 Document (3.366 seconds)

BMRB: 6191

Sample

Related entities 1. SecA, : 1 : 22 : 67 : 3 : 30 entities Detail

Interaction partners 1. SecA, : 94 interactors Detail

Experiments performed 2 experiments Detail

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Sample #4