BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.5 % (735 of 803)	94.0 % (394 of 419)	88.7 % (282 of 318)	89.4 % (59 of 66)
Backbone	90.1 % (366 of 406)	92.9 % (130 of 140)	89.2 % (181 of 203)	87.3 % (55 of 63)
Sidechain	90.7 % (417 of 460)	92.8 % (259 of 279)	87.1 % (155 of 178)	100.0 % (3 of 3)
Aromatic	61.5 % (48 of 78)	64.1 % (25 of 39)	56.8 % (21 of 37)	100.0 % (2 of 2)
Methyl	98.3 % (59 of 60)	100.0 % (30 of 30)	96.7 % (29 of 30)

1. Src Homology-3 domain

GKDPKFEAAY DFPGSGSSSE LPLKKGDIVF ISRDEPSGWS LAKLLDGSKE GWVPTAYMTP YKDTRNTVPV

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2BTT, Strand ID: A Detail

Release date

2004-11-07

Citation

1H, 15N and 13C assigned chemical shifts for the Myosin type I SH3 domain (Myo3) from saccharomyces cerevisiae
Musi, V., Birdsall, B., Pastore, A.

Related entities 1. Src homology-3 domain, : 1 : 1 : 4 : 146 entities Detail

Interaction partners 1. Src homology-3 domain, : 65 interactors Detail

More details for 65 interactors identified by 10 citations

Experiments performed 11 experiments Detail

1 1H-15N NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

2 1H-15N TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

3 HNHA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

4 HNHB

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

5 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

6 CBCANH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

7 HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

8 HCCH TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

9 2D NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

10 2D COSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

11 2D TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian UNITY (500 MHz)

#3 Model Varian UNITY (600 MHz)

#4 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology-3 domain	[U-95% 13C; U-90% 15N]		0.6 mM

Sample #2

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Src Homology-3 domain	[U-90% 15N]		0.6 mM

Sample #3

Temperature 298 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Src Homology-3 domain			0.6 mM

nullKeywords SH3, mys3, yeast, myosin type I, NMR

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Found 1 Document (3.918 seconds)

BMRB: 6197

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Src homology-3 domain, : 1 : 1 : 4 : 146 entities Detail

Interaction partners 1. Src homology-3 domain, : 65 interactors Detail

Experiments performed 11 experiments Detail

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