BMRB: 6200

Assignment of the minicollagen 1 cysteine rich domain

Authors

Meier, S., Grzesiek, S.

Assembly

MCRD 1

Entity

1. MCRD 1 (polymer, Thiol state: all disulfide bound), 24 monomers, 2592.172 Da Detail

PCPPVCVAQC VPTCPQYCCP AKRK

Formula weight

2592.172 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS18:SG
2	disulfide	sing	1:CYS6:SG	1:CYS14:SG
3	disulfide	sing	1:CYS10:SG	1:CYS19:SG

Source organism

Hydra

Exptl. method

NMR

Refine. method

simulated annealing with torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.7 %, Completeness (bb): 81.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.7 % (244 of 269)	99.3 % (143 of 144)	77.1 % (81 of 105)	100.0 % (20 of 20)
Backbone	81.8 % (108 of 132)	100.0 % (42 of 42)	66.7 % (48 of 72)	100.0 % (18 of 18)
Sidechain	99.4 % (160 of 161)	99.0 % (101 of 102)	100.0 % (57 of 57)	100.0 % (2 of 2)
Aromatic	100.0 % (8 of 8)	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. MCRD

PCPPVCVAQC VPTCPQYCCP AKRK

Show sample condition

Sample

Pressure 1 atm, Temperature 288 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	MCRD			5 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 1SP7, Strand ID: A Detail

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Release date

2004-05-03

Citation

Determination of a high-precision NMR structure of the minicollagen cysteine rich domain from Hydra and characterization of its disulfide bond formation
Meier, S., Haussinger, D., Pokidysheva, E., Bachinger, H.P., Grzesiek, S.
FEBS Lett. (2004), 569, 112-116, PubMed 15225618 , DOI 10.1016/j.febslet.2004.05.034 , Abstract

Related entities 1. MCRD 1, : 1 : 1 : 4 entities Detail

Experiments performed 5 experiments Detail