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BMRB: 6208

1TUZ

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Diacylglycerol kinase alpha

Authors

Liu, G., Shao, Y., Xiao, R., Acton, T., Montelione, G.T., Szyperski, T.

Assembly

hr532 monomer

Entity

1. hr532 monomer (polymer, Thiol state: all free), 118 monomers, 13486.13 Da Detail

MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA KYVQGDAIGY EGFQQFLKIY LEVDNVPRHL SLALFQSFET GHCLNETNVT KDVVCLNDVS CYFSLLEGGR PEDKLEWS

Formula weight

13486.13 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.7 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.7 % (1259 of 1404)	97.7 % (712 of 729)	78.0 % (428 of 549)	94.4 % (119 of 126)
Backbone	84.6 % (594 of 702)	97.1 % (234 of 241)	72.5 % (251 of 346)	94.8 % (109 of 115)
Sidechain	95.2 % (773 of 812)	98.0 % (478 of 488)	91.1 % (285 of 313)	90.9 % (10 of 11)
Aromatic	79.7 % (110 of 138)	94.2 % (65 of 69)	64.7 % (44 of 68)	100.0 % (1 of 1)
Methyl	98.4 % (120 of 122)	98.4 % (60 of 61)	98.4 % (60 of 61)

1. Ca.CD2

MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA KYVQGDAIGY EGFQQFLKIY LEVDNVPRHL SLALFQSFET GHCLNETNVT KDVVCLNDVS CYFSLLEGGR PEDKLEWS

Show sample condition

Sample

Temperature 298 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Ca.CD2	[U-13C; U-15N]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	MES		20 mM
6	D2O		5 %
7	NaN3		0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	external	direct
¹⁵N	DSS	na	null	null
¹³C	DSS	na	null	null

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	external	direct
¹⁵N	DSS	na	null	null
¹³C	DSS	na	null	null

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	external	direct
¹⁵N	DSS	na	null	null
¹³C	DSS	na	null	null

Referencing offset: 0.06 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1TUZ, Strand ID: A Detail

Coupling constant

36 J values in 1 lists
Temperature 298 K, pH 6.5 (±0.1) Detail

Release date

2006-01-10

Citation

1H, 13C and 15N Assigned Chemical Shifts for Diacylglycerol kinase alpha
Liu, G., Shao, Y., Xiao, R., Acton, T., Montelione, G.T., Szyperski, T.
J. Biomol. NMR

Related entities 1. hr532 monomer, : 1 : 2 : 22 entities Detail

Interaction partners 1. hr532 monomer, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 9 experiments Detail

1 15N 2D HSQC