BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.3 % (686 of 804)	84.0 % (352 of 419)	84.4 % (266 of 315)	97.1 % (68 of 70)
Backbone	97.3 % (389 of 400)	97.1 % (132 of 136)	97.5 % (193 of 198)	97.0 % (64 of 66)
Sidechain	76.9 % (360 of 468)	77.7 % (220 of 283)	75.1 % (136 of 181)	100.0 % (4 of 4)
Aromatic	23.9 % (21 of 88)	36.4 % (16 of 44)	11.4 % (5 of 44)
Methyl	90.0 % (54 of 60)	90.0 % (27 of 30)	90.0 % (27 of 30)

1. N-terminal domain of At3g03410.1

SSAKRVFEKF DKNKDGKLSL DEFREVALAF SPYFTQEDIV KFFEEIDVDG NGELNADEFT SCIEKML

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	N-terminal domain of At3g03410.1	[U-15N]	1 mM
2	DTT		5 mM
3	Bis-Tris		20 mM
4	CaCl2		5 mM
5	DSS		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.85 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1TIZ, Strand ID: A Detail

RDC

55 RDC values in 1 lists
Field strength (¹H) 0.0 MHz, Temperature 298 (±1) K, pH 6.4 (±0.2) Detail

Release date

2004-06-29

Citation

Letter to the Editor: Solution Structure of a Calmodulin-Like Calcium-Binding Domain from Arabidopsis thaliana
Song, J., Zhao, Q., Thao, S., Frederick, R.O., Markley, J.L.
J. Biomol. NMR (2004), 30, 451-456, PubMed , DOI:

Related entities 1. N-domain of At3g03410.1, : 1 : 2 : 521 entities Detail

Experiments performed 5 experiments Detail

1 HNCACB

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	N-terminal domain of At3g03410.1	[U-15N]	1 mM
2	DTT		5 mM
3	Bis-Tris		20 mM
4	CaCl2		5 mM
5	DSS		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
6	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
7	DTT		5 mM
8	Bis-Tris		20 mM
9	CaCl2		5 mM
10	DSS		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.2), Details Dissolved in 5% Pentaethylene Glycol Monododecyl Ether (weakly aligned medium)

#	Name	Isotope labeling	Concentration
11	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
12	DTT		5 mM
13	Bis-Tris		20 mM
14	CaCl2		5 mM
15	DSS		1 mM
16	PEG		5 %

2 HN(CO)CACB

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	N-terminal domain of At3g03410.1	[U-15N]	1 mM
2	DTT		5 mM
3	Bis-Tris		20 mM
4	CaCl2		5 mM
5	DSS		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
6	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
7	DTT		5 mM
8	Bis-Tris		20 mM
9	CaCl2		5 mM
10	DSS		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.2), Details Dissolved in 5% Pentaethylene Glycol Monododecyl Ether (weakly aligned medium)

#	Name	Isotope labeling	Concentration
11	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
12	DTT		5 mM
13	Bis-Tris		20 mM
14	CaCl2		5 mM
15	DSS		1 mM
16	PEG		5 %

3 N15 edited NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	N-terminal domain of At3g03410.1	[U-15N]	1 mM
2	DTT		5 mM
3	Bis-Tris		20 mM
4	CaCl2		5 mM
5	DSS		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
6	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
7	DTT		5 mM
8	Bis-Tris		20 mM
9	CaCl2		5 mM
10	DSS		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.2), Details Dissolved in 5% Pentaethylene Glycol Monododecyl Ether (weakly aligned medium)

#	Name	Isotope labeling	Concentration
11	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
12	DTT		5 mM
13	Bis-Tris		20 mM
14	CaCl2		5 mM
15	DSS		1 mM
16	PEG		5 %

4 N15 edited TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	N-terminal domain of At3g03410.1	[U-15N]	1 mM
2	DTT		5 mM
3	Bis-Tris		20 mM
4	CaCl2		5 mM
5	DSS		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
6	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
7	DTT		5 mM
8	Bis-Tris		20 mM
9	CaCl2		5 mM
10	DSS		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.2), Details Dissolved in 5% Pentaethylene Glycol Monododecyl Ether (weakly aligned medium)

#	Name	Isotope labeling	Concentration
11	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
12	DTT		5 mM
13	Bis-Tris		20 mM
14	CaCl2		5 mM
15	DSS		1 mM
16	PEG		5 %

5 IPAP-HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	N-terminal domain of At3g03410.1	[U-15N]	1 mM
2	DTT		5 mM
3	Bis-Tris		20 mM
4	CaCl2		5 mM
5	DSS		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Concentration
6	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
7	DTT		5 mM
8	Bis-Tris		20 mM
9	CaCl2		5 mM
10	DSS		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.2), Details Dissolved in 5% Pentaethylene Glycol Monododecyl Ether (weakly aligned medium)

#	Name	Isotope labeling	Concentration
11	N-terminal domain of At3g03410.1	[U-15N; U-13C]	1 mM
12	DTT		5 mM
13	Bis-Tris		20 mM
14	CaCl2		5 mM
15	DSS		1 mM
16	PEG		5 %

null

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BMRB: 6209

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. N-domain of At3g03410.1, : 1 : 2 : 521 entities Detail

Experiments performed 5 experiments Detail

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