BMRB: 6216

Solution structure of the third zinc finger domain of FOG-1

Authors

Simpson, R.J.Y., Lee, S.H.Y., Bartle, N., Matthews, J.M., Mackay, J.P., Crossley, M.

Assembly

Zinc finger protein ZFPM1

Entity

1. ZFPM1 (polymer, Thiol state: free and other bound), 35 monomers, 3806.401 Da Detail

GSSGKRPFVC RICLSAFTTK ANCARHLKVH TDTLS

2. ZN (non-polymer), 65.409 Da

Total weight

3871.8098 Da

Max. entity weight

3806.401 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS10:SG	2:ZN1:ZN
2	na	sing	1:CYS13:SG	2:ZN1:ZN
3	na	sing	1:HIS26:NE2	2:ZN1:ZN
4	na	sing	1:HIS30:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 87.6 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.6 % (176 of 201)	87.6 % (176 of 201)
Backbone	94.4 % (67 of 71)	94.4 % (67 of 71)
Sidechain	83.8 % (109 of 130)	83.8 % (109 of 130)
Aromatic	71.4 % (10 of 14)	71.4 % (10 of 14)
Methyl	94.7 % (18 of 19)	94.7 % (18 of 19)

1. ZFPM1

GSSGKRPFVC RICLSAFTTK ANCARHLKVH TDTLS

Show sample condition

Sample #1

Pressure 1 atm, Temperature 275 K, pH 5.6

#	Name	Isotope labeling	Type	Concentration
1	ZFPM1			0.27 mM
2	ZnSO4			1 mM
3	TCEP			2 mM
4	H2O			95 %
5	D2O			5 %

Sample #2

Pressure 1 atm, Temperature 275 K, pH 5.6

#	Name	Isotope labeling	Type	Concentration
6	ZFPM1	[U-15N]		0.245 mM
7	ZnSO4			1 mM
8	TCEP			2 mM
9	H2O			95 %
10	D2O			5 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1SRK, Strand ID: A Detail

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Coupling constant

31 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 275 K, pH 5.6 Detail

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Pressure 1 atm, Temperature 275 K, pH 5.6

List #1 coupling_constants_set_1, Field strength (¹H) 600 MHz

Release date

2005-02-07

Citation

A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3
Simpson, R.J., Lee, S.H.Y., Bartle, N., Sum, E.Y., Visvader, J.E., Matthews, J.M., Mackay, J.P., Crossley, M.
J. Biol. Chem. (2004), 279, 39789-39797, PubMed 15234987 , DOI 10.1074/jbc.M404130200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. ZFPM1, : 1 : 937 entities Detail

Interaction partners 1. ZFPM1, : 2 interactors Detail

Experiments performed 4 experiments Detail