BMRBj - BMREntryMaster

Found 1 Document (3.782 seconds)

BMRB: 6221

1XSF

Backbone 1H, 13C, and 15N Chemical Shift Assignments of the Resuscitation Promoting Factor (Rpf) domain of M. tuberculosis

Authors

Cohen-Gonsaud, M., Barthe, P., Keep, N.H., Roumestand, C.

Assembly

RpfBc

Entity

1. RpfBc (polymer, Thiol state: all disulfide bound), 108 monomers, 11347.49 Da Detail

NVVVTPAHEA VVRVGTKPGT EVPPVIDGSI WDAIAGCEAG GNWAINTGNG YYGGVQFDQG TWEANGGLRY APRADLATRE EQIAVAEVTR LRQGWGAWPV CAARAGAR

Formula weight

11347.49 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS37:SG	1:CYS101:SG

Source organism

Mycobacterium tuberculosis H37Rv

Exptl. method

NMR

Refine. method

molecular dynamics in explicit solvent

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.3 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.3 % (984 of 1153)	94.7 % (557 of 588)	69.3 % (311 of 449)	100.0 % (116 of 116)
Backbone	98.1 % (624 of 636)	98.2 % (222 of 226)	97.4 % (300 of 308)	100.0 % (102 of 102)
Sidechain	73.6 % (448 of 609)	92.5 % (335 of 362)	42.5 % (99 of 233)	100.0 % (14 of 14)
Aromatic	54.1 % (53 of 98)	83.7 % (41 of 49)	15.9 % (7 of 44)	100.0 % (5 of 5)
Methyl	64.1 % (82 of 128)	95.3 % (61 of 64)	32.8 % (21 of 64)

1. Rpf domain

NVVVTPAHEA VVRVGTKPGT EVPPVIDGSI WDAIAGCEAG GNWAINTGNG YYGGVQFDQG TWEANGGLRY APRADLATRE EQIAVAEVTR LRQGWGAWPV CAARAGAR

Show sample condition

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -1.13 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1XSF, Strand ID: A Detail

Release date

2004-05-27

Citation

1H, 15N and 13C Chemical Shifts Assignments of the Resuscitation Promoting Factor domain of Rv1009 from Mycobacterium tuberculosis
Cohen-Gonsaud, M., Barthe, P., Pommier, F., Harris, R., Driscoll, P.C., Keep, N.H., Roumestand, C.
J. Biomol. NMR (2004), 30, 373-374, PubMed , DOI:

Related entities 1. RpfBc, : 1 : 4 : 5 entities Detail

Experiments performed 8 experiments Detail

1 HNCA

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

2 HN(CO)CA

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

3 HNCACB

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

4 CBCA(CO)NH

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

5 HNCO

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

6 HN(CA)CO

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

7 1H-15N NOESY

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

8 1H-15N TOCSY

Instrument

Model Bruker Avance (500 MHz) Details A cryogenic probe equipped the 500 MHz spectrometer.

Sample #1

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Rpf domain	[U-95% 15N]	0.5 mM
2	Na-Acetate		25 mM
3	beta-mercaptoethanol		5 mM

Sample #2

Temperature 293 (±0.2) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Concentration
4	Rpf domain	[U-95% 13C; U-95% 15N]	0.5 mM
5	Na-Acetate		25 mM
6	beta-mercaptoethanol		5 mM

nullKeywords M. tuberculosis, NMR assignments, Resuscitation, Rpf

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (3.782 seconds)

BMRB: 6221

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RpfBc, : 1 : 4 : 5 entities Detail

Experiments performed 8 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2