BMRB: 6222

Solution Structure of Kurtoxin

Authors

Lee, C.W., Min, H.J., Cho, E.M., Kohno, T., Eu, Y.J., Kim, J.I.

Assembly

Kurtoxin

Entity

1. Kurtoxin (polymer, Thiol state: all disulfide bound), 63 monomers, 7394.423 Da Detail

KIDGYPVDYW NCKRICWYNN KYCNDLCKGL KADSGYCWGW TLSCYCQGLP DNARIKRSGR CRA

Formula weight

7394.423 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS12:SG	1:CYS61:SG
2	disulfide	sing	1:CYS16:SG	1:CYS37:SG
3	disulfide	sing	1:CYS23:SG	1:CYS44:SG
4	disulfide	sing	1:CYS27:SG	1:CYS46:SG

Source organism

Parabuthus transvaalicus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 99.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.2 % (383 of 394)	97.2 % (383 of 394)
Backbone	99.2 % (129 of 130)	99.2 % (129 of 130)
Sidechain	96.2 % (254 of 264)	96.2 % (254 of 264)
Aromatic	91.7 % (44 of 48)	91.7 % (44 of 48)
Methyl	100.0 % (20 of 20)	100.0 % (20 of 20)

1. scorpion toxin

KIDGYPVDYW NCKRICWYNN KYCNDLCKGL KADSGYCWGW TLSCYCQGLP DNARIKRSGR CRA

Show sample condition

Sample

Pressure 1 atm, Temperature 288 (±1) K, pH 4.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	scorpion toxin	[U-15N; U-13C]		1 mM
2	CD3CN			25 %
3	D2O			10 %
4	H2O			65 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1T1T, Strand ID: A Detail

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Release date

2004-05-31

Citation

Solution structure of kurtoxin: a gating modifier selective for Cav3 voltage-gated Ca(2+) channels
Lee, C.W., Min, H.J., Cho, E.M., Kohno, T., Eu, Y.J., Kim, J.I.
Biochemistry (2012), 51, 1862-1873, PubMed 22329781 , DOI 10.1021/bi201633j , Abstract

Related entities 1. Kurtoxin, : 1 : 1 : 137 entities Detail

Experiments performed 6 experiments Detail