BMRB: 6226

1H, 13C, and 15N Chemical Shift Assignments for the C-terminal domain of EW29

Authors

Hemmi, H.

Assembly

EW29 monomer

Entity

1. EW29 monomer (polymer, Thiol state: all free), 132 monomers, 14858.59 Da Detail

MKPKFFYIKS ELNGKVLDIE GQNPAPGSKI ITWDQKKGPT AVNQLWYTDQ QGVIRSKLND FAIDASHEQI ETQPFDPNNP KRAWIVSGNT IAQLSDRDIV LDIIKSDKEA GAHICAWKQH GGPNQKFIIE SE

Formula weight

14858.59 Da

Source organism

Lumbricus terrestris

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 95.8 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.8 % (1514 of 1581)	95.2 % (791 of 831)	97.2 % (587 of 604)	93.2 % (136 of 146)
Backbone	96.6 % (750 of 776)	95.5 % (253 of 265)	97.9 % (379 of 387)	95.2 % (118 of 124)
Sidechain	95.4 % (885 of 928)	95.1 % (538 of 566)	96.8 % (329 of 340)	81.8 % (18 of 22)
Aromatic	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (59 of 59)	100.0 % (4 of 4)
Methyl	95.5 % (126 of 132)	97.0 % (64 of 66)	93.9 % (62 of 66)

1. C-terminal domain of EW29

MKPKFFYIKS ELNGKVLDIE GQNPAPGSKI ITWDQKKGPT AVNQLWYTDQ QGVIRSKLND FAIDASHEQI ETQPFDPNNP KRAWIVSGNT IAQLSDRDIV LDIIKSDKEA GAHICAWKQH GGPNQKFIIE SE

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.1 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of EW29	[U-15N; U-13C]		0.9 mM

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LACS Plot; CA

Referencing offset: 0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.66 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RST, Strand ID: A Detail

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Release date

2004-12-15

Citation

(1)H, (13)C, and (15)N chemical shift assignment of the C-terminal 15 kDa domain of a novel galactose-binding protein from the earthworm Lumbricus terrestris
Hemmi, H., Kuno, A., Ito, S., Suzuki, R., Kaneko, S., Hasegawa, T., Hirabayashi, J., Kasai, K.
J. Biomol. NMR (2004), 30, 377-378, PubMed 15756471 , DOI 10.1007/s10858-005-1845-6 , Abstract

Related entities 1. EW29 monomer, : 1 : 2 : 3 entities Detail

Experiments performed 1 experiments Detail