BMRBj - BMREntryMaster

Found 1 Document (3.266 seconds)

BMRB: 6235

2N1T

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the SNARE complex

Authors

Chen, X., Rizo, J.

Assembly

SNARE complex

Entity

1. Synaptobrevin2 SNARE motif (polymer, Thiol state: not present), 65 monomers, 7617.484 Da Detail

NRRLQQTQAQ VDEVVDIMRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE TSAAKLKRKY WWKNL

2. Syntaxin 1A SNARE motif (polymer, Thiol state: not present), 63 monomers, 7397.289 Da Detail

ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD TKK

3. SNAP-25B N-terminal SNARE motif (polymer, Thiol state: not present), 73 monomers, 8544.447 Da Detail

LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLDRV EEGMNHINQD MKEAEKNLKD LGW

4. SNAP-25B C-terminal SNARE motif (polymer, Thiol state: not present), 64 monomers, 7459.247 Da Detail

ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLW

Total weight

31018.469 Da

Max. entity weight

8544.447 Da

Source organism

Rattus norvegicus , Homo sapiens

Exptl. method

NMR

Refine. method

MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 24.2 %, Completeness: 7.1 %, Completeness (bb): 12.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	7.1 % (222 of 3118)	5.2 % (85 of 1644)	6.7 % (78 of 1172)	19.5 % (59 of 302)
Backbone	12.6 % (201 of 1590)	12.6 % (68 of 539)	9.4 % (74 of 786)	22.3 % (59 of 265)
Sidechain	1.7 % (30 of 1784)	1.5 % (17 of 1105)	2.0 % (13 of 642)	0.0 % (0 of 37)
Aromatic	0.0 % (0 of 112)	0.0 % (0 of 56)	0.0 % (0 of 52)	0.0 % (0 of 4)
Methyl	5.0 % (14 of 278)	5.8 % (8 of 139)	4.3 % (6 of 139)

1. Synaptobrevin2 SNARE motif

NRRLQQTQAQ VDEVVDIMRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE TSAAKLKRKY WWKNL

2. Syntaxin 1A SNARE motif

ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD TKK

3. SNAP-25B N-terminal SNARE motif

LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLDRV EEGMNHINQD MKEAEKNLKD LGW

4. SNAP-25B C-terminal SNARE motif

ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLW

Show sample condition

Sample

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Synaptobrevin2 SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
2	Syntaxin 1A SNARE motif		0.3 mM
3	SNAP-25B N-terminal SNARE motif		0.3 mM
4	SNAP-25B C-terminal SNARE motif		0.3 mM

Chem. Shift Complete2

Sequence coverage: 23.8 %, Completeness: 7.8 %, Completeness (bb): 12.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	7.8 % (488 of 6236)	6.2 % (204 of 3288)	7.1 % (166 of 2344)	19.5 % (118 of 604)
Backbone	12.9 % (411 of 3180)	13.2 % (142 of 1078)	9.6 % (151 of 1572)	22.3 % (118 of 530)
Sidechain	2.7 % (97 of 3568)	2.8 % (62 of 2210)	2.7 % (35 of 1284)	0.0 % (0 of 74)
Aromatic	4.0 % (9 of 224)	6.3 % (7 of 112)	1.9 % (2 of 104)	0.0 % (0 of 8)
Methyl	4.9 % (27 of 556)	5.4 % (15 of 278)	4.3 % (12 of 278)

1. Synaptobrevin2 SNARE motif

NRRLQQTQAQ VDEVVDIMRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE TSAAKLKRKY WWKNL

2. Syntaxin 1A SNARE motif

ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD TKK

3. SNAP-25B N-terminal SNARE motif

LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLDRV EEGMNHINQD MKEAEKNLKD LGW

4. SNAP-25B C-terminal SNARE motif

ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLW

Show sample condition

Sample

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Synaptobrevin2 SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
2	Syntaxin 1A SNARE motif		0.3 mM
3	SNAP-25B N-terminal SNARE motif		0.3 mM
4	SNAP-25B C-terminal SNARE motif		0.3 mM

Chem. Shift Complete3

Sequence coverage: 30.6 %, Completeness: 8.8 %, Completeness (bb): 14.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	8.8 % (824 of 9354)	6.9 % (340 of 4932)	8.1 % (285 of 3516)	22.0 % (199 of 906)
Backbone	14.4 % (688 of 4770)	14.7 % (237 of 1617)	10.8 % (254 of 2358)	24.8 % (197 of 795)
Sidechain	3.1 % (168 of 5352)	3.1 % (103 of 3315)	3.3 % (63 of 1926)	1.8 % (2 of 111)
Aromatic	2.7 % (9 of 336)	4.2 % (7 of 168)	1.3 % (2 of 156)	0.0 % (0 of 12)
Methyl	5.2 % (43 of 834)	5.5 % (23 of 417)	4.8 % (20 of 417)

1. Synaptobrevin2 SNARE motif

NRRLQQTQAQ VDEVVDIMRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE TSAAKLKRKY WWKNL

2. Syntaxin 1A SNARE motif

ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD TKK

3. SNAP-25B N-terminal SNARE motif

LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLDRV EEGMNHINQD MKEAEKNLKD LGW

4. SNAP-25B C-terminal SNARE motif

ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLW

Show sample condition

Sample

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Synaptobrevin2 SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
2	Syntaxin 1A SNARE motif		0.3 mM
3	SNAP-25B N-terminal SNARE motif		0.3 mM
4	SNAP-25B C-terminal SNARE motif		0.3 mM

Chem. Shift Complete4

Sequence coverage: 24.9 %, Completeness: 8.6 %, Completeness (bb): 14.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	8.6 % (1069 of 12472)	6.6 % (436 of 6576)	7.9 % (369 of 4688)	21.9 % (264 of 1208)
Backbone	14.2 % (902 of 6360)	14.3 % (309 of 2156)	10.5 % (331 of 3144)	24.7 % (262 of 1060)
Sidechain	2.9 % (206 of 7136)	2.9 % (127 of 4420)	3.0 % (77 of 2568)	1.4 % (2 of 148)
Aromatic	3.3 % (15 of 448)	4.9 % (11 of 224)	1.9 % (4 of 208)	0.0 % (0 of 16)
Methyl	4.7 % (52 of 1112)	5.2 % (29 of 556)	4.1 % (23 of 556)

1. Synaptobrevin2 SNARE motif

NRRLQQTQAQ VDEVVDIMRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE TSAAKLKRKY WWKNL

2. Syntaxin 1A SNARE motif

ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD TKK

3. SNAP-25B N-terminal SNARE motif

LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLDRV EEGMNHINQD MKEAEKNLKD LGW

4. SNAP-25B C-terminal SNARE motif

ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLW

Show sample condition

Sample

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Synaptobrevin2 SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
2	Syntaxin 1A SNARE motif		0.3 mM
3	SNAP-25B N-terminal SNARE motif		0.3 mM
4	SNAP-25B C-terminal SNARE motif		0.3 mM

Protein Blocks Logo

Calculated from 5 models in PDB: 2N1T, Strand ID: A, B, C, D Detail

Release date

2005-03-06

Citation

Are neuronal SNARE proteins Ca2+ sensors?
Chen, X., Tang, J., Sudhof, T.C., Rizo, J.
J. Mol. Biol. (2005), 347, 145-158, PubMed 15733924 , DOI 10.1016/j.jmb.2005.01.024 , Abstract

Related entities 1. Synaptobrevin2 SNARE motif, : 6 : 1 : 11 : 168 entities Detail

Related entities 2. Syntaxin 1A SNARE motif, : 1 : 34 : 2 : 4 : 157 entities Detail

Related entities 3. SNAP-25B N-terminal SNARE motif, : 1 : 3 : 25 : 80 entities Detail

Related entities 4. SNAP-25B C-terminal SNARE motif, : 1 : 1 : 36 : 95 entities Detail

Interaction partners 1. Synaptobrevin2 SNARE motif, : 14 : 1 interactors Detail

More details for 15 interactors identified by 12 citations

Interaction partners 2. Syntaxin 1A SNARE motif, : 5 : 1 interactors Detail

More details for 6 interactors identified by 3 citations

Interaction partners 3. SNAP-25B N-terminal SNARE motif, : 14 : 1 interactors Detail

More details for 15 interactors identified by 11 citations

Interaction partners 4. SNAP-25B C-terminal SNARE motif, : 16 : 1 interactors Detail

More details for 17 interactors identified by 12 citations

Experiments performed 2 experiments Detail

1 1H-15N NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Synaptobrevin2 SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
2	Syntaxin 1A SNARE motif		0.3 mM
3	SNAP-25B N-terminal SNARE motif		0.3 mM
4	SNAP-25B C-terminal SNARE motif		0.3 mM

Sample #2

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	Synaptobrevin2 SNARE motif		0.3 mM
6	Syntaxin 1A SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
7	SNAP-25B N-terminal SNARE motif		0.3 mM
8	SNAP-25B C-terminal SNARE motif		0.3 mM

Sample #3

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	Synaptobrevin2 SNARE motif		0.3 mM
10	Syntaxin 1A SNARE motif		0.3 mM
11	SNAP-25B N-terminal SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
12	SNAP-25B C-terminal SNARE motif		0.3 mM

Sample #4

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	Synaptobrevin2 SNARE motif		0.3 mM
14	Syntaxin 1A SNARE motif		0.3 mM
15	SNAP-25B N-terminal SNARE motif		0.3 mM
16	SNAP-25B C-terminal SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM

2 Transverse-relaxation optimized spectroscopy (TROSY) based HNCA and TROSY based HN(CO)CA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Synaptobrevin2 SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
2	Syntaxin 1A SNARE motif		0.3 mM
3	SNAP-25B N-terminal SNARE motif		0.3 mM
4	SNAP-25B C-terminal SNARE motif		0.3 mM

Sample #2

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	Synaptobrevin2 SNARE motif		0.3 mM
6	Syntaxin 1A SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
7	SNAP-25B N-terminal SNARE motif		0.3 mM
8	SNAP-25B C-terminal SNARE motif		0.3 mM

Sample #3

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	Synaptobrevin2 SNARE motif		0.3 mM
10	Syntaxin 1A SNARE motif		0.3 mM
11	SNAP-25B N-terminal SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM
12	SNAP-25B C-terminal SNARE motif		0.3 mM

Sample #4

Temperature 305 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
13	Synaptobrevin2 SNARE motif		0.3 mM
14	Syntaxin 1A SNARE motif		0.3 mM
15	SNAP-25B N-terminal SNARE motif		0.3 mM
16	SNAP-25B C-terminal SNARE motif	[U-2H; U-13C; U-15N]	0.3 mM

nullKeywords membrane fusion, SNARE proteins, the core complex, Ca2+ binding sites, TROSY-based NMR

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (3.266 seconds)

BMRB: 6235

Sample

Sample

Sample

Sample

Related entities 1. Synaptobrevin2 SNARE motif, : 6 : 1 : 11 : 168 entities Detail

Related entities 2. Syntaxin 1A SNARE motif, : 1 : 34 : 2 : 4 : 157 entities Detail

Related entities 3. SNAP-25B N-terminal SNARE motif, : 1 : 3 : 25 : 80 entities Detail

Related entities 4. SNAP-25B C-terminal SNARE motif, : 1 : 1 : 36 : 95 entities Detail

Interaction partners 1. Synaptobrevin2 SNARE motif, : 14 : 1 interactors Detail

Interaction partners 2. Syntaxin 1A SNARE motif, : 5 : 1 interactors Detail

Interaction partners 3. SNAP-25B N-terminal SNARE motif, : 14 : 1 interactors Detail

Interaction partners 4. SNAP-25B C-terminal SNARE motif, : 16 : 1 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4