BMRBj - BMREntryMaster

Found 1 Document (1.637 seconds)

BMRB: 6236

1TKN

Backbone 1H, 13C, and 15N Chemical Shift Assignments for CAPPD*, an Independently Folded Extracellular Domain of Human Amyloid-beta Precursor Protein

Authors

Dulubova, I., Ho, A., Huryeva, I., Sudhof, T.C., Rizo, J.

Assembly

APP770

Entity

1. APP770 (polymer, Thiol state: not present), 117 monomers, 13952.85 Da Detail

RVEAMLNDRR RLALENYITA LQAVPPRPRH VFNMLKKYVR AEQKDRQHTL KHFEHVRMVD PKKAAQIRSQ VMTHLRVIYE RMNQSLSLLY NVPAVAEEIQ DEVDELLQKE QNYSDDV

Formula weight

13952.85 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.1 %, Completeness (bb): 99.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	99.1 % (1423 of 1436)	98.9 % (751 of 759)	99.1 % (545 of 550)	100.0 % (127 of 127)
Backbone	99.3 % (687 of 692)	100.0 % (229 of 229)	98.6 % (346 of 351)	100.0 % (112 of 112)
Sidechain	99.1 % (853 of 861)	98.5 % (522 of 530)	100.0 % (316 of 316)	100.0 % (15 of 15)
Aromatic	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)
Methyl	100.0 % (136 of 136)	100.0 % (68 of 68)	100.0 % (68 of 68)

1. Amyloid-beta Precursor Protein

RVEAMLNDRR RLALENYITA LQAVPPRPRH VFNMLKKYVR AEQKDRQHTL KHFEHVRMVD PKKAAQIRSQ VMTHLRVIYE RMNQSLSLLY NVPAVAEEIQ DEVDELLQKE QNYSDDV

Show sample condition

Sample

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Amyloid-beta Precursor Protein	[U-99% 15N]	0.85 mM
2	sodium phosphate		50 mM
3	NaCl		250 mM
4	Guanidinium Chloride		300 mM
5	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.85 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1TKN, Strand ID: A Detail

Release date

2004-08-31

Citation

Three-dimensional structure of an independently folded extracellular domain of human amyloid-beta precursor protein
Dulubova, I., Ho, A., Huryeva, I., Sudhof, T.C., Rizo, J.
Biochemistry (2004), 43, 9583-9588, PubMed 15274612 , DOI 10.1021/bi049041o , Abstract

Related entities 1. APP770, : 1 : 8 : 3 : 7 : 29 entities Detail

Interaction partners 1. APP770, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 5 experiments Detail

1 3D 1H-15N NOESY-HSQC and TOCSY-HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Amyloid-beta Precursor Protein	[U-99% 15N]	0.85 mM
2	sodium phosphate		50 mM
3	NaCl		250 mM
4	Guanidinium Chloride		300 mM
5	D2O		5 %

Sample #2

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	Amyloid-beta Precursor Protein	[U-99% 15N; U-13C]	0.85 mM
7	sodium phosphate		50 mM
8	NaCl		250 mM
9	Guanidinium Chloride		300 mM
10	D2O		5 %

2 HNCO, HNCACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Amyloid-beta Precursor Protein	[U-99% 15N]	0.85 mM
2	sodium phosphate		50 mM
3	NaCl		250 mM
4	Guanidinium Chloride		300 mM
5	D2O		5 %

Sample #2

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	Amyloid-beta Precursor Protein	[U-99% 15N; U-13C]	0.85 mM
7	sodium phosphate		50 mM
8	NaCl		250 mM
9	Guanidinium Chloride		300 mM
10	D2O		5 %

3 H(C)(CO)NH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Amyloid-beta Precursor Protein	[U-99% 15N]	0.85 mM
2	sodium phosphate		50 mM
3	NaCl		250 mM
4	Guanidinium Chloride		300 mM
5	D2O		5 %

Sample #2

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	Amyloid-beta Precursor Protein	[U-99% 15N; U-13C]	0.85 mM
7	sodium phosphate		50 mM
8	NaCl		250 mM
9	Guanidinium Chloride		300 mM
10	D2O		5 %

4 (H)C(CO)NH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Amyloid-beta Precursor Protein	[U-99% 15N]	0.85 mM
2	sodium phosphate		50 mM
3	NaCl		250 mM
4	Guanidinium Chloride		300 mM
5	D2O		5 %

Sample #2

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	Amyloid-beta Precursor Protein	[U-99% 15N; U-13C]	0.85 mM
7	sodium phosphate		50 mM
8	NaCl		250 mM
9	Guanidinium Chloride		300 mM
10	D2O		5 %

5 HCCH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Amyloid-beta Precursor Protein	[U-99% 15N]	0.85 mM
2	sodium phosphate		50 mM
3	NaCl		250 mM
4	Guanidinium Chloride		300 mM
5	D2O		5 %

Sample #2

Temperature 300 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
6	Amyloid-beta Precursor Protein	[U-99% 15N; U-13C]	0.85 mM
7	sodium phosphate		50 mM
8	NaCl		250 mM
9	Guanidinium Chloride		300 mM
10	D2O		5 %

nullKeywords Amyloid-beta Precursor Protein, Alzheimer's disease, CAPPD, CAPPD*, four alpha-helices, three-helix bundle

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Found 1 Document (1.637 seconds)

BMRB: 6236

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. APP770, : 1 : 8 : 3 : 7 : 29 entities Detail

Interaction partners 1. APP770, : 2 interactors Detail

Experiments performed 5 experiments Detail

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