BMRBj - BMREntryMaster

Found 1 Document (1.774 seconds)

BMRB: 6238

1TOT

Complete 1H, 15N amd 13C Chemical Shift Assignments of the murine CBP ZZ domain (a.a. 1700-1751)

Authors

Legge, G.B., Hambly, D., Trinh, T., Martinez-Yamout, M.A., Dyson, H.JANE., Wright, P.E.

Assembly

ZZ domain of CBP

Entity

1. ZZ (polymer, Thiol state: all other bound), 52 monomers, 6218.968 Da Detail

GQDRFVYTCN ECKHHVETRW HCTVCEDYDL CINCYNTKSH THKMVKWGLG LD

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

6349.7856 Da

Max. entity weight

6218.968 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS9:SG	2:ZN1:ZN
2	na	sing	1:CYS12:SG	2:ZN1:ZN
3	na	sing	1:CYS31:SG	2:ZN1:ZN
4	na	sing	1:CYS34:SG	2:ZN1:ZN
5	na	sing	1:CYS22:SG	2:ZN1:ZN
6	na	sing	1:CYS25:SG	2:ZN1:ZN
7	na	sing	1:HIS40:NE2	2:ZN1:ZN
8	na	sing	1:HIS42:ND1	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (587 of 608)	98.7 % (309 of 313)	93.7 % (222 of 237)	96.6 % (56 of 58)
Backbone	95.8 % (299 of 312)	98.1 % (105 of 107)	94.1 % (144 of 153)	96.2 % (50 of 52)
Sidechain	97.4 % (336 of 345)	99.0 % (204 of 206)	94.7 % (126 of 133)	100.0 % (6 of 6)
Aromatic	92.3 % (72 of 78)	100.0 % (39 of 39)	83.8 % (31 of 37)	100.0 % (2 of 2)
Methyl	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)

1. ZZ

GQDRFVYTCN ECKHHVETRW HCTVCEDYDL CINCYNTKSH THKMVKWGLG LD

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	ZZ	[U-13C; U-15N]	2.3 mM
2	Zinc Chloride		50 uM
3	Salt		150 mM
4	Tris		20 mM
5	DTT		2 mM
6	D2O		5 % v/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	H2O	protons	external	direct
¹⁵N	H2O	N	external	indirect
¹³C	H2O	C	external	indirect
¹¹³Cd	CdCl2	Cadmium (zinc substitute)	external	direct

Referencing offset: 1.64 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	H2O	protons	external	direct
¹⁵N	H2O	N	external	indirect
¹³C	H2O	C	external	indirect
¹¹³Cd	CdCl2	Cadmium (zinc substitute)	external	direct

Referencing offset: 1.64 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	H2O	protons	external	direct
¹⁵N	H2O	N	external	indirect
¹³C	H2O	C	external	indirect
¹¹³Cd	CdCl2	Cadmium (zinc substitute)	external	direct

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	H2O	protons	external	direct
¹⁵N	H2O	N	external	indirect
¹³C	H2O	C	external	indirect
¹¹³Cd	CdCl2	Cadmium (zinc substitute)	external	direct

Referencing offset: 0.56 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1TOT, Strand ID: A Detail

Release date

2004-11-11

Citation

ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module
Legge, G.B., Martinez-Yamout, M.A., Hambly, D.M., Trinh, T., Lee, B.M., Dyson, H.JANE., Wright, P.E.
J. Mol. Biol. (2004), 343, 1081-1093, PubMed 15476823 , DOI 10.1016/j.jmb.2004.08.087 , Abstract

Related entities 1. ZZ, : 1 : 5 : 3 : 21 entities Detail

Interaction partners 1. ZZ, : 31 interactors Detail

More details for 31 interactors identified by 24 citations

Experiments performed 17 experiments Detail

1 1H15N HSQC