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BMRB: 6244

1TM9

Novel all alpha-helical fold for gene target gi3844938 from Mycoplasma genitalium

Authors

Shi, J., Pelton, J.G., Wemmer, D.E.

Assembly

Mycoplasma Genitalium gi3844938

Entity

1. Mycoplasma Genitalium gi3844938 (polymer, Thiol state: not reported), 137 monomers, 15703.54 Da Detail

MEQNNIKEQL ISFFNQACST HQERLDFICS TRESDTFSSV DVPLEPIKNI IEITKDENQQ IEITKIAVNN IKTLSSVGAT GQYMASFFST NSEPAIIFCV IYFLYHFGFL KDNNKKQIIK KAYETIADNI ADYLNEN

Formula weight

15703.54 Da

Source organism

Mycoplasma genitalium G37

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.0 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.0 % (1370 of 1650)	79.6 % (681 of 856)	87.0 % (556 of 639)	85.8 % (133 of 155)
Backbone	97.8 % (798 of 816)	97.1 % (266 of 274)	98.3 % (401 of 408)	97.8 % (131 of 134)
Sidechain	72.4 % (701 of 968)	70.6 % (411 of 582)	78.9 % (288 of 365)	9.5 % (2 of 21)
Aromatic	23.6 % (35 of 148)	36.5 % (27 of 74)	10.8 % (8 of 74)
Methyl	99.4 % (153 of 154)	98.7 % (76 of 77)	100.0 % (77 of 77)

1. gi3844938

MEQNNIKEQL ISFFNQACST HQERLDFICS TRESDTFSSV DVPLEPIKNI IEITKDENQQ IEITKIAVNN IKTLSSVGAT GQYMASFFST NSEPAIIFCV IYFLYHFGFL KDNNKKQIIK KAYETIADNI ADYLNEN

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

Protein Blocks Logo

Calculated from 26 models in PDB: 1TM9, Strand ID: A Detail

Release date

2004-08-03

Citation

Novel all alpha-helical fold for gene target gi3844938 from Mycoplasma genitalium
Shi, J., Pelton, J.G., Yokota, H., Kim, R., Wemmer, D.

Related entities 1. Mycoplasma Genitalium gi3844938, : 1 : 1 : 1 entities Detail

Experiments performed 15 experiments Detail

1 15N-HSQC

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

2 HNCA

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

3 CBCA(CO)NH

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

4 HNCACB

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

5 HNCACO

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

6 HACANH

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

7 hC CONH

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

8 Hc CONH

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

9 HCCH-TOCSY

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

10 15N-NOESY-HSQC

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

11 15N-TOCSY-HSQC

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

12 2D NOESY

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

13 2D TOCSY

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

14 2D 13C Double half-filtered TOCSY

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

15 2D 13C Dboule half-filtered NOESY

Instrument

Bruker Bruker - 500 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	gi3844938	[U-93% 13C; U-98% 15N]		1.0 mM
2	KPO4			25 mM
3	NaCl			100 mM
4	EDTA			1 mM
5	TCEP			1 mM

nullKeywords protein fold, BSGC, structural genomics, nmr