BMRB: 6247

Solution structure of C-terminal domain of NifU-like protein from Oryza sativa

Authors

Kumeta, H., Ogura, K., Asayama, M., Katoh, S., Katoh, E., Inagaki, F.

Assembly

NifU1

Entity

1. NifU1 (polymer, Thiol state: not present), 74 monomers, 8094.624 Da Detail

MLELNEENVE KVLNEIRPYL AGTGGGGLQF LMIKGPIVKV RLTGPAAVVR TVRIAVSKKL REKIPSIQIV QLLS

Formula weight

8094.624 Da

Source organism

Oryza sativa

Exptl. method

NMR

Refine. method

distance geometr,y simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.8 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.8 % (838 of 884)	93.1 % (431 of 463)	96.5 % (333 of 345)	97.4 % (74 of 76)
Backbone	96.1 % (419 of 436)	96.0 % (145 of 151)	95.8 % (206 of 215)	97.1 % (68 of 70)
Sidechain	94.0 % (484 of 515)	91.7 % (286 of 312)	97.5 % (192 of 197)	100.0 % (6 of 6)
Aromatic	88.9 % (16 of 18)	77.8 % (7 of 9)	100.0 % (9 of 9)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. NifU1

MLELNEENVE KVLNEIRPYL AGTGGGGLQF LMIKGPIVKV RLTGPAAVVR TVRIAVSKKL REKIPSIQIV QLLS

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NifU1	[U-15N]		0.8 mM
2	NaPi			20 mM
3	NaCl			100 mM
4	beta-ME			2.5 mM
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 0.49 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1TH5, Strand ID: A Detail

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Release date

2005-10-19

Citation

Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli
Katoh, S., Murata, K., Kubota, Y., Kumeta, H., Ogura, K., Inagaki, F., Asayama, M., Katoh, E.
Protein Expr. Purif. (2005), 43, 149-156, PubMed 15978837 , DOI 10.1016/j.pep.2005.04.019 , Abstract

Related entities 1. NifU1, : 1 : 2 : 43 entities Detail

Experiments performed 2 experiments Detail