BMRB: 6262

NMR structure of WW domains (WW3-4) from Suppressor of Deltex

Authors

Fedoroff, O.Y., Avis, J.M., Golovanov, A.P., Baron, M., Townson, S.A.

Assembly

CG4244-PB

Entity

1. CG4244-PB (polymer, Thiol state: not present), 88 monomers, 10266.15 Da Detail

GSPEFHMDAL GPLPDGWEKK IQSDNRVYFV NHKNRTTQWE DPRTQGQEVS LINEGPLPPG WEIRYTAAGE RFFVDHNTRR TTFEDPRP

Formula weight

10266.15 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 71.0 %, Completeness (bb): 95.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	71.0 % (458 of 645)	68.1 % (377 of 554)	89.0 % (81 of 91)
Backbone	95.7 % (242 of 253)	94.8 % (165 of 174)	97.5 % (77 of 79)
Sidechain	55.1 % (216 of 392)	55.8 % (212 of 380)	33.3 % (4 of 12)
Aromatic	63.3 % (38 of 60)	63.2 % (36 of 57)	66.7 % (2 of 3)
Methyl	65.6 % (21 of 32)	65.6 % (21 of 32)

1. CG4244-PB

GSPEFHMDAL GPLPDGWEKK IQSDNRVYFV NHKNRTTQWE DPRTQGQEVS LINEGPLPPG WEIRYTAAGE RFFVDHNTRR TTFEDPRP

Show sample condition

Sample

Pressure 1 atm, Temperature 288 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	CG4244-PB	[U-15N]		0.5 mM
2	Arg/Glu			50 mM
3	H2O			90 %
4	D2O			10 %
5	Na/KCl			150 mM

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Protein Blocks Logo

Calculated from 7 models in PDB: 1TK7, Strand ID: A Detail

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Release date

2004-09-19

Citation

The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex
Fedoroff, O.Y., Townson, S.A., Golovanov, A.P., Baron, M., Avis, J.M.
J. Biol. Chem. (2004), 279, 34991-35000, PubMed 15173166 , DOI 10.1074/jbc.M404987200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. CG4244-PB, : 1 : 2 : 231 entities Detail

Interaction partners 1. CG4244-PB, : 9 interactors Detail

Experiments performed 5 experiments Detail