BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	80.1 % (735 of 918)	85.6 % (409 of 478)	72.9 % (263 of 361)	79.7 % (63 of 79)
Backbone	76.3 % (348 of 456)	89.0 % (138 of 155)	65.9 % (151 of 229)	81.9 % (59 of 72)
Sidechain	85.4 % (457 of 535)	83.9 % (271 of 323)	88.8 % (182 of 205)	57.1 % (4 of 7)
Aromatic	65.8 % (50 of 76)	65.8 % (25 of 38)	66.7 % (24 of 36)	50.0 % (1 of 2)
Methyl	92.9 % (78 of 84)	92.9 % (39 of 42)	92.9 % (39 of 42)

1. PUB14

GSPEFPEYFR CPISLELMKD PVIVSTGQTY ERSSIQKWLD AGHKTCPKSQ ETLLHAGLTP NYVLKSLIAL WCESNGIE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1T1H, Strand ID: A Detail

Release date

2005-02-07

Citation

Structure and biochemical function of a prototypical Arabidopsis U-box domain
Andersen, P., Kragelund, B.B., Olsen, A.N., Larsen, F.H., Chua, N., Poulsen, F.M., Skriver, K.
J. Biol. Chem. (2004), 279, 40053-40061, PubMed 15231834 , DOI 10.1074/jbc.M405057200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q8VZ40 released on 2005-03-29
Title PUB14_ARATH Entity U-box domain-containing protein 14

Related entities 1. armadillo repeat and U-box containing protein, : 1 : 2 : 218 entities Detail

Interaction partners 1. armadillo repeat and U-box containing protein, : 15 interactors Detail

More details for 15 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 15N HSQC

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

2 HNCACB

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

3 CBCA(CO)NH

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

4 HNCO

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

5 HN(CA)CO

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

6 (HB)CB(CGCD)HD

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

7 3D 15N-separated TOCSY

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

8 3D 15N-separated NOESY

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

9 3D 13C-separated TOCSY

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

10 3D 13C-separated NOESY

Instrument

Model Varian UNITY INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	PUB14	[U-15N; U-13C]	0.9 mM
2	sodium phosphate		20 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	D20		10 %
6	H20		90 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	PUB14	[U-15N; U-13C]	0.6 mM
8	sodium phosphate		20 mM
9	NaCl		0.15 M
10	DTT		1 mM
11	D20		10 %
12	H20		90 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	PUB14	[U-15N]	0.6 mM
14	sodium phosphate		20 mM
15	NaCl		0.15 M
16	DTT		1 mM
17	D20		10 %
18	H20		90 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
19	PUB14	[U-15N; U-13C]	0.4 mM
20	sodium phosphate		20 mM
21	NaCl		0.15 M
22	DTT		1 mM
23	D20		99.96 %
24	H20		0.04 %

nullKeywords ARABIDOPSIS, E3 LIGASE, NMR, UBIQUITIN LIGASE, U-BOX

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BMRB: 6265

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Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. armadillo repeat and U-box containing protein, : 1 : 2 : 218 entities Detail

Interaction partners 1. armadillo repeat and U-box containing protein, : 15 interactors Detail

Experiments performed 10 experiments Detail

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Sample #1