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BMRB: 6266

1TL4

Solution structure of Cu(I) HAH1

Authors

Anastassopoulou, I., Banci, L., Bertini, I., Cantini, F., Katsari, E., Rosato, A.

Assembly

Copper transport protein ATOX1

Entity

1. Copper transport protein ATOX1 (polymer, Thiol state: all free), 68 monomers, 7401.556 Da Detail

MPKHEFSVDM TCGGCAEAVS RVLNKLGGVK YDIDLPNKKV CIESEHSMDT LLATLKKTGK TVSYLGLE

2. CU1 (non-polymer), 63.546 Da

Total weight

7465.102 Da

Max. entity weight

7401.556 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 88.2 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	88.2 % (411 of 466)	86.7 % (345 of 398)	97.1 % (66 of 68)
Backbone	96.1 % (198 of 206)	95.7 % (134 of 140)	97.0 % (64 of 66)
Sidechain	79.6 % (207 of 260)	79.5 % (205 of 258)	100.0 % (2 of 2)
Aromatic	47.1 % (8 of 17)	47.1 % (8 of 17)
Methyl	95.0 % (38 of 40)	95.0 % (38 of 40)

1. Copper transport protein ATOX1

MPKHEFSVDM TCGGCAEAVS RVLNKLGGVK YDIDLPNKKV CIESEHSMDT LLATLKKTGK TVSYLGLE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1TL4, Strand ID: A Detail

Release date

2005-02-07

Citation

Solution structure of the apo and copper(I)-loaded human metallochaperone HAH1
Anastassopoulou, I., Banci, L., Bertini, I., Cantini, F., Katsari, E., Rosato, A.
Biochemistry (2004), 43, 13046-13053, PubMed 15476398 , DOI 10.1021/bi0487591 , Abstract

Related entities 1. Copper transport protein ATOX1, : 1 : 13 : 5 : 130 entities Detail

Interaction partners 1. Copper transport protein ATOX1, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 CBCANH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

2 CBCACONH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

3 HNCO

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

4 HN(CA)CO

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

5 (H)CCH TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

6 HNHA

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

7 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

8 2D NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

9 2D TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

10 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Copper transport protein ATOX1	[U-15N]		1.0 mM
2	sodium acetate			10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
5	Copper transport protein ATOX1	[U-95% 13C; U-98% 15N]		1.0 mM
6	DTT			4 mM
7	phosphate buffer			100 mM
8	H2O			90 %
9	D2O			10 %

nullKeywords copper protein, copper chaperone, Menkes, Wilson