BMRB: 6268

CBP TAZ1 Domain

Authors

De Guzman, R.N., Wojciak, J.M., Martinez-Yamout, M.A., Dyson, H., wright, P.E.

Assembly

CBP TAZ1

Entity

1. CBP TAZ1 domain (polymer, Thiol state: all other bound), 100 monomers, 11256.98 Da Detail

ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

11453.207 Da

Max. entity weight

11256.98 Da

Entity Connection

na 12 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:HIS23:NE2	2:ZN1:ZN
2	na	sing	1:CYS27:SG	2:ZN1:ZN
3	na	sing	1:CYS40:SG	2:ZN1:ZN
4	na	sing	1:CYS45:SG	2:ZN1:ZN
5	na	sing	1:HIS54:NE2	2:ZN1:ZN
6	na	sing	1:CYS58:SG	2:ZN1:ZN
7	na	sing	1:CYS64:SG	2:ZN1:ZN
8	na	sing	1:CYS69:SG	2:ZN1:ZN
9	na	sing	1:HIS78:NE2	2:ZN1:ZN
10	na	sing	1:CYS82:SG	2:ZN1:ZN
11	na	sing	1:CYS87:SG	2:ZN1:ZN
12	na	sing	1:CYS90:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics by DYANA, simulated annealing and molecular dynamics by AMBER

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 75.3 %, Completeness (bb): 79.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.3 % (866 of 1150)	78.4 % (476 of 607)	68.0 % (295 of 434)	87.2 % (95 of 109)
Backbone	79.8 % (471 of 590)	96.5 % (191 of 198)	62.6 % (186 of 297)	98.9 % (94 of 95)
Sidechain	73.8 % (485 of 657)	69.7 % (285 of 409)	85.0 % (199 of 234)	7.1 % (1 of 14)
Aromatic	29.5 % (13 of 44)	31.8 % (7 of 22)	23.8 % (5 of 21)	100.0 % (1 of 1)
Methyl	95.9 % (94 of 98)	95.9 % (47 of 49)	95.9 % (47 of 49)

1. CBP TAZ1 domain

ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.9 (±0.2), Details ~1 mM N15 TAZ1 10 mM Tris pH 6.9 50 mM NaCl 2 mM DTT

#	Name	Isotope labeling	Type	Concentration
1	CBP TAZ1 domain	[U-13C; U-15N]		1 mM
2	Tris			10 mM
3	NaCl			50 mM
4	DTT			2 mM

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1U2N, Strand ID: A Detail

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Release date

2005-02-20

Citation

CBP/p300 TAZ1 domain forms a structured scaffold for ligand binding
De Guzman, R.N., Wojciak, J.M., Martinez-Yamout, M.A., Dyson, H., Wright, P.E.
Biochemistry (2005), 44, 490-497, PubMed 15641773 , DOI 10.1021/bi048161t , Abstract

Related entities 1. CBP TAZ1 domain, : 1 : 11 : 1 : 63 entities Detail

Interaction partners 1. CBP TAZ1 domain, : 78 interactors Detail

Experiments performed 1 experiments Detail